Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/69495
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dc.contributor.authorDung, T.D.en_US
dc.contributor.authorChang, H.C.en_US
dc.contributor.authorBinh, T.V.en_US
dc.contributor.authorLee, M.R.en_US
dc.contributor.authorTsai, C.H.en_US
dc.contributor.authorTsai, F.J.en_US
dc.contributor.authorKuo, W.W.en_US
dc.contributor.authorChen, L.M.en_US
dc.contributor.authorHuang, C.Y.en_US
dc.date2012zh_TW
dc.date.accessioned2014-06-11T05:58:24Z-
dc.date.available2014-06-11T05:58:24Z-
dc.identifier.issn1107-3756zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/69495-
dc.description.abstractHepatocellular carcinoma is a common type of cancer that is usually associated with poor prognosis. In this study, we examined the in vitro and in vivo mechanisms of the traditional Vietnamese herb Zanthoxylum avicennae on the inhibition of HA22T human hepatocellular carcinoma cell proliferation. HA22T cells were treated with different concentrations of Zanthoxylum avicennae extracts (YBBEs) and analyzed with the MTT assay, western blot analysis, flow cytometry, siRNA transfection assays and co-immunoprecipitation assay. Additionally, the HA22T-implanted xenograft nude mouse model was applied to confirm the cellular effects. YBBEs showed a strong inhibition of HA22T cell viability in a dose-dependent manner and significantly reduced cell proliferation-related proteins as well as induced cell cycle arrest in the G2/M phase. Protein phosphatase 2A (PP2A) siRNA or okadaic acid totally blocked YBBE-mediated cell proliferation inhibition. In addition, an HA22T-implanted nude mouse model further confirmed that YBBEs inhibit HA22T tumor cell growth and downregulate the survival and cell cycle regulating proteins, as well as activate the PP2A protein. Our findings indicate that the inhibition of HA22T cell proliferation by YBBEs is mediated through PP2A activation.en_US
dc.language.isoen_USzh_TW
dc.relationInternational Journal of Molecular Medicineen_US
dc.relation.ispartofseriesInternational Journal of Molecular Medicine, Volume 29, Issue 6, Page(s) 1045-1052.en_US
dc.relation.urihttp://dx.doi.org/10.3892/ijmm.2012.938en_US
dc.subjecthepatocellular carcinomaen_US
dc.subjectZanthoxylum avicennae (Ying Bu Bo)en_US
dc.subjectproteinen_US
dc.subjectphosphatase 2Aen_US
dc.subjectcell proliferationen_US
dc.subjectnude mouse modelen_US
dc.subjectp53 ubiquitinationen_US
dc.subjectapoptosisen_US
dc.subjectmdm2en_US
dc.subjectmiceen_US
dc.subjectepidemiologyen_US
dc.subjectpathogenesisen_US
dc.subjectgrowthen_US
dc.subjectgeneen_US
dc.titleZanthoxylum avicennae extracts inhibit cell proliferation through protein phosphatase 2A activation in HA22T human hepatocellular carcinoma cells in vitro and in vivoen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.3892/ijmm.2012.938zh_TW
item.openairetypeJournal Article-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextnone-
item.fulltextno fulltext-
item.cerifentitytypePublications-
item.languageiso639-1en_US-
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