Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/69770
標題: Exploration of the Relationship Between the Tumor Burden and the Concentration of Vascular Endothelial Growth Factor in Liver-Cancer-Bearing Animals Using Immunomagnetic Reduction Assay
作者: Huang, K.W.
Yang, S.Y.
Yu, C.Y.
Chieh, J.J.
Yang, C.C.
Horng, H.E.
Hong, C.Y.
Yang, H.C.
Wu, C.C.
關鍵字: Immunomagnetic Reduction;Vascular Endothelial Growth Factor;Magnetic;Nanoparticle;Hepatocellular Carcinoma;Animal Model;human hepatocellular-carcinoma;magnetic nanoparticles;factor vegf;marker;expression;virus
Project: Journal of Biomedical Nanotechnology
期刊/報告no:: Journal of Biomedical Nanotechnology, Volume 7, Issue 4, Page(s) 535-541.
摘要: 
To achieve early-stage diagnosis, a high-sensitivity assay method is needed. As a biomarker, vascular endothelial growth factor (VEGF) has played a growing role in diagnosing and treating hepatocellular carcinoma (HCC). In this work, an immunomagnetic reduction (IMR) through bio-functionalized magnetic nanoparticles and a high-temperature superconducting-quantum-interference-device magnetometer were utilized for quantitative detection of low-concentration VEGF in serum from rats with HCC. The precision and accuracy of IMR on VEGF were characterized. Further, the results of assaying VEGF in the serum of rats were compared with those of using enzyme-linked immunosorbant assay (ELISA). It was found the correlations between the detected VEGF concentration in the rat serum and tumor burdens were 0.99 and 0.90 for IMR and ELISA, respectively, within the range from 2 pg/ml to 8000 pg/ml of VEGF concentration.
URI: http://hdl.handle.net/11455/69770
ISSN: 1550-7033
DOI: 10.1166/jbn.2011.1321
Appears in Collections:期刊論文

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