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標題: Preparative separation and purification of epigallocatechin gallate from green tea extracts using a silica adsorbent containing beta-cyclodextrin
作者: Lai, S.M.
Gu, J.Y.
Huang, B.H.
Chang, C.M.J.
Lee, W.L.
關鍵字: Silica adsorbent containing beta-cyclodextrin;Green tea extracts;Catechins;Preparative separation and purification;Column;chromatography;solid-phase extraction;agarose-gel media;bonded silica;chromatographic-separation;liquid-chromatography;stationary-phase;phenol compounds;catechins;retention;particles
Project: Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
期刊/報告no:: Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, Volume 887, Page(s) 112-121.
A silica adsorbent containing beta-cyclodextrin (beta-CD) has been developed and used for the separation and purification of epigallocatechin gallate (EGCG) from the green tea extracts. The batch adsorption experiments demonstrated that, the beta-CD bonded silica adsorbent possessed excellent adsorption equilibrium capacity (>55 mg/g adsorbent) and adsorption ratio (>95%) for EGCG compared to the other tea catechins and caffeine. The excellent adsorption capacity and selectivity for EGCG are attributed to the specific interactions between beta-CD and EGCG. The preparative separation and purification performance of EGCG on the beta-CD bonded silica column (220 mm L x 15 mm id., 40-63 mu m) was then evaluated. The column was operated in the polar organic mode using methanol/acetonitrile/acetic acid as the mobile phase and eluted under a three-step gradient elution program. The sample was dissolved in acetonitrile and loaded on a preparative scale of about 0.8 mg/g adsorbent. Under the optimal chromatographic conditions, the target compound, EGCG, being the most retained species, was obtained at a purity of about 90% with a recovery of about 90%. The productivity of EGCG was about 6 mg per injection, which can be further increased by scaling-up the chromatographic system. (c) 2012 Elsevier BM. All rights reserved.
ISSN: 1570-0232
DOI: 10.1016/j.jchromb.2012.01.019
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