Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/69925
DC FieldValueLanguage
dc.contributor.authorWang, H.W.en_US
dc.contributor.authorLiou, K.T.en_US
dc.contributor.authorWang, Y.H.en_US
dc.contributor.authorLu, C.K.en_US
dc.contributor.authorLin, Y.L.en_US
dc.contributor.authorLee, I.J.en_US
dc.contributor.authorHuang, S.T.en_US
dc.contributor.authorTsai, Y.H.en_US
dc.contributor.authorCheng, Y.C.en_US
dc.contributor.authorLin, H.J.en_US
dc.contributor.authorShen, Y.C.en_US
dc.date2011zh_TW
dc.date.accessioned2014-06-11T05:59:06Z-
dc.date.available2014-06-11T05:59:06Z-
dc.identifier.issn0378-8741zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/69925-
dc.description.abstractEthnopharmacological relevance: Bu-yang Huan-wu decoction (BHD) is a famous traditional Chinese medicine formula that has been used clinically in Asia to treat stroke-induced disability for centuries, but the underlying neuroprotective mechanisms are not fully understood. Aim of the study: In this study, we aim to investigate the mechanisms of action using an integrative neurofunctional and broad genomics approach. Materials and methods: Male ICR mice were subjected to an acute ischemic stroke by inducing a middle cerebral ischemic/reperfusion (CI/R) injury. To examine whether BHD could extend the lifespan of mice with a stroke, we used oral administration of BHD (0.5 and 1.0 g/kg) twice daily starting from 2h after ischemia and compared this with vehicle control treatments, recombinant tissue-type plasminogen activator (rt-PA, 10 mg/kg, iv.), and MK-801 (0.2 mg/kg, i.p.). An integrative neurofunctional and genomic approach was performed to elucidate the underlying molecular mechanisms of BHD. Results: More than 80% of the mice died within 2 days after stroke induction in the vehicle control treatment group. However, the survival rates and life-spans of mice treated with BHD, rt-PA and MK-801 were significantly enhanced as compared to the vehicle-treated CI/R group in all three cases. Mice treated with BHD (1.0 g/kg) showed the greatest protective effect across all groups. BHD successfully restored brain function, ameliorated the cerebral infarction, and significantly improved the neurological deficits of the mice with a stroke. BHD also reduced inflammation, oxidative stress, and apoptosis, as well as improved neurogenesis. The molecular impacts of BHD were assessed by genome-wide transcriptome analysis using brains from the CUR mice. The results showed a total of 377 ischemia-induced probe-sets that were significantly influenced by BHD including 93 probe-sets that were commonly more abundant in BHD-treated and sham mice, and another 284 ischemia-induced probe sets that were suppressed by BHD. Mining the functional modules and genetic networks of these 377 genes revealed a significant upregulation of neuroprotective genes associated with neurogenesis (6 genes) and nervous system development (9 genes), and a significant down-regulation of destructive genes associated with the induction of inflammation (14 genes), apoptosis (15 genes), angiogenesis (11 genes) and blood coagulation (7 genes) by BHD. Conclusions: Our results suggested that BHD is able to protect mice against stroke and extend lifespan primarily through a significant down-regulation of genes involved in inflammation, apoptosis, angiogenesis and blood coagulation, as well as an up-regulation of genes mediating neurogenesis and nervous system development. The changes in expression after treatment with BHD are beneficial after ischemic stroke. (C) 2011 Elsevier Ireland Ltd. All rights reserved.en_US
dc.language.isoen_USzh_TW
dc.relationJournal of Ethnopharmacologyen_US
dc.relation.ispartofseriesJournal of Ethnopharmacology, Volume 138, Issue 1, Page(s) 22-33.en_US
dc.relation.urihttp://dx.doi.org/10.1016/j.jep.2011.06.033en_US
dc.subjectApoptosisen_US
dc.subjectBu-yang Huan-wu decoctionen_US
dc.subjectCerebral ischemia-reperfusionen_US
dc.subject(CI/R)en_US
dc.subjectFunctional modules and genetic networksen_US
dc.subjectGenome-wideen_US
dc.subjecttranscriptome analysisen_US
dc.subjectInfarctionen_US
dc.subjectInflammationen_US
dc.subjectMiceen_US
dc.subjectMicroarrayen_US
dc.subjectNeurogenesisen_US
dc.subjectOxidative stressen_US
dc.subjectPositron emission tomography (PET)en_US
dc.subjectNeurological deficitsen_US
dc.subjectfocal cerebral-ischemiaen_US
dc.subjectkappa-ben_US
dc.subjectneuronal injuryen_US
dc.subjectinfarct volumeen_US
dc.subjectbrain-injuryen_US
dc.subjectactivationen_US
dc.subjectratsen_US
dc.subjectaccumulationen_US
dc.titleDeciphering the neuroprotective mechanisms of Bu-yang Huan-wu decoction by an integrative neurofunctional and genomic approach in ischemic stroke miceen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1016/j.jep.2011.06.033zh_TW
item.languageiso639-1en_US-
item.grantfulltextnone-
item.cerifentitytypePublications-
item.fulltextno fulltext-
item.openairetypeJournal Article-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
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