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作者: Chou, P.Y.
Huang, G.J.
Cheng, H.C.
Wu, C.H.
Chien, Y.C.
Chen, J.S.
Huang, M.H.
Hsu, K.J.
Sheu, M.J.
關鍵字: formalin test;antioxidant activity;rich alga;carotenoids;bardawil;rats;mice;cells;microalgae;edema
Project: Journal of Food Biochemistry
期刊/報告no:: Journal of Food Biochemistry, Volume 34, Issue 6, Page(s) 1288-1302.
This study investigated the analgesic and anti-inflammatory effects of an ethanol extract of Dunaliella salina Teod. (Chlorophyceae) (EDS) in Imprinting Control Region mice. Standard all-trans-beta-carotene and the amount of all-trans-beta-carotene in an EDS were analyzed by high-performance liquid chromatography (HPLC). In HPLC analysis, the fingerprint chromatogram of EDS was established. Both all-trans-beta-carotene and EDS showed similar peaks at the retention time of 24 min. This implied that EDS contained the active ingredient all-trans-beta-carotene. Treatment of animals with EDS significantly inhibited the numbers of acetic acid-induced writhing responses at doses of 0.5 g/kg (P < 0.01), 1.0 g/kg (P < 0.001) and 2.0 g/kg (P < 0.001). This inhibitory effect of EDS (1.0 and 2.0 g/kg) on acetic acid-induced writhings was similar to that of the positive control indomethacin (10 mg/kg) (P < 0.001). EDS did not significantly inhibit the formalin-induced pain in the early phase; however, at doses of 0.1 g/kg (P < 0.01), 0.5, 1.0 and 2.0 g/kg, EDS significantly inhibited the formalin-induced pain in the late phase (P < 0.001). Finally, EDS at doses of 1.0 and 2.0 g/kg also inhibited the development of paw edema induced by lambda-carrageenan (carrageenan). EDS (1.0 and 2.0 mg/kg) decreased the level of nitiric oxide (NO) in edematous paw tissue and in serum level, and diminished the level of serum tumor necrosis factor-alpha (TNF-alpha) at the fifth hour after carrageenan injection. Based on these findings, EDS probably exerts anti-inflammatory effects by suppressing TNF-alpha and NO. These results suggest that EDS might be a potential pharmacological analgesic and anti-inflammatory agent. PRACTICAL APPLICATIONS Dunaliella salina (Dunal) Teod. is a valuable beta-carotene source. D. salina is cultivated in a great quantity in the southern part of Taiwan. In order to find its potential application for this product, the analgesic and anti-inflammatory effects were investigated. It was found that D. salina could attenuate the acetic acid-induced writhings and formalin-induced analgesia. We also demonstrate that D. salina could lessen the carrageenan-induced paw edema. Also, the anti-inflammatory effects verified that nitric oxide and TNF-alpha could play an import role.
ISSN: 0145-8884
DOI: 10.1111/j.1745-4514.2010.00389.x
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