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標題: PCR primers for the detection of staphylococcal enterotoxins K, L, and M and survey of staphylococcal enterotoxin types in Staphylococcus aureus isolates from food poisoning cases in Taiwan
作者: Cihang, Y.C.
Chang, L.T.
Lin, C.W.
Yang, C.Y.
Tsen, H.Y.
關鍵字: polymerase-chain-reaction;toxic-shock-syndrome;pathogenicity island;exfoliative toxins;genes;superantigens;strains;identification;samples;prevalence
Project: Journal of Food Protection
期刊/報告no:: Journal of Food Protection, Volume 69, Issue 5, Page(s) 1072-1079.
Staphylococcal enterotoxins (SEs) are important causative agents in gastroenteritidis and food poisoning cases. They are serologically grouped into five major classical types, i.e., SEA, SEB, SEC, SED, and SEE. In addition, new SEs, such as SEG through SEM, have recently been identified and characterized. In an attempt to survey the distribution of classical and new SEs in organisms responsible for staphylococcal infections in Taiwan, we developed PCR primers for the genes that define the SEK, SEL, and SEM types. Bacterial strains other than sek, set, and sem Stapkylococcus aureus, including strains of other Staphylococcus species, did not generate any false-positive results when examined with these primers. The expression potential for the sek, set, and sent types were also determined by reverse transcription-PCR. Together with the PCR primers specific for the classical SEs and other new SEs, including SEG, SEH, SEI, and SEJ, we surveyed the SE genes in S. aureus strains isolated from food poisoning cases. For 147 S. aureus isolates originating from food poisoning cases, 109 (74.1%) were positive for one or more SE genes. Of them, the major classical enterotoxin type was sea (28.6%), followed by seb (20.4%), sec (8.2%), and sed (2.0%). For the new SE types, sei (30.6%) was detected the most often, followed by sek (18.4%), sem (12.9%), and set (8.2%). Also, 64 (43.5%) of the total bacterial strains had more than one enterotoxin gene.
ISSN: 0362-028X
Appears in Collections:期刊論文

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