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|標題:||Use of a multiplex PCR system for the simultaneous detection of heat labile toxin I and heat stable toxin II genes of enterotoxigenic Escherichia coli in skim milk and porcine stool||作者:||Tsen, H.Y.
|關鍵字:||polymerase chain-reaction;enzymatic amplification;colony;hybridization;dna-sequences;identification;infection;specimens;diarrhea;strains;samples||Project:||Journal of Food Protection||期刊/報告no：:||Journal of Food Protection, Volume 61, Issue 2, Page(s) 141-145.||摘要:||
Enterotoxigenic Escherichia coli (ETEC) strains which produce heat labile and/or heat stable toxins (LT and ST) may cause diarrhea in humans and farm animals. Using PCR primers specific for the LT I and ST II genes, a multiplex PCR system which allows detection of LT I-and ST II-producing ETEC strains was developed. When skim milk was used for a PCR assay, it was found that if target cells in the sample were precultured in MacConkey broth for 8 h prior to PCR as few as 10(0) cells per mi of the sample could be detected. Without the preculture step, 10(4) CFU of target cells per 0.2 g of porcine stool specimen were required to generate visible PCR products. The multiplex PCR system can be used for rapid testing of fecal specimens, food and possibly environmental samples for the presence of ETEC strains.
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