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|標題:||Identification and characterization of RNA-binding activities of avian reovirus non-structural protein sigma NS||作者:||Yin, H.S.
|關鍵字:||double-stranded-rna;virus;polypeptides;strain||Project:||Journal of General Virology||期刊/報告no：:||Journal of General Virology, Volume 79, Page(s) 1411-1413.||摘要:||
Cytoplasmic extracts prepared from avian reovirus (ARV) strain S1133-infected chicken embryo fibroblasts were examined for the presence of RNA-binding proteins in order to identify and characterize ARV RNA-binding proteins. Analysis of binding activity to poly(A)Sepharose indicated that infected cells contained significant amounts of a protein that co-migrated with ARV protein sigma NS present in total virus-infected cell extracts. Determination of the N-terminal amino acid sequence of several peptide fragments generated by V8 protease digestion of the poly(A)-Sepharose-purified protein confirmed that this viral protein was sigma NS. Competition assays showed that single-stranded RNA from the unrelated avian pathogen infectious bursal disease virus was able to compete for binding of sigma NS to poly(A)-Sepharose. These data suggest that ARV sigma NS binds to single-stranded RNA in a nucleotide sequence non-specific manner and is functionally similar to its counterpart specified by mammalian reovirus.
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