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標題: Development of the reversible PGA immobilization by using the immobilized metal ion affinity membrane
作者: Chen, C.I.
Ko, Y.M.
Lien, W.L.
Lin, Y.H.
Li, I.T.
Chen, C.H.
Shieh, C.J.
Liu, Y.C.
關鍵字: Immobilized metal affinity membrane;Enzyme immobilization;Penicillin G;acylase;Regeneration process;Coordination immobilization;penicillin-g acylase;protein adsorption;escherichia-coli;purification;chromatography;supports
Project: Journal of Membrane Science
期刊/報告no:: Journal of Membrane Science, Volume 401, Page(s) 33-39.
In this study, an immobilized metal ion affinity membrane (IMAM) was prepared and used to immobilize penicillin G acylase (PGA). The stability of storage of the immobilized PGA membrane (IPM) was tested. When stored in deionized water (DI) at 4 degrees C, only 19% residual activity of IPM was retained for 10 days. However, when stored in 10 mM phosphate buffer (PB, pH 8) with 0.1% NaN3, the IPM can retain 99% of its activity for a 10-day reaction and storage test. The feasibility of the IPM regeneration after a long period of reaction was investigated. When using 100 mM EDTA as the stripping solution, the re-immobilized capacity for PGA is only 52% of the original one after 4 regenerations. When the regeneration process was modified by using the stripping solution (300 mM NaCl, 25 mM EDTA, 20 mM PB, pH 8) for 30 min, then immersed in 0.5 M HCl and 0.5 M NaOH respectively for 10 min, the resulting IMAM can reserve 99% PGA immobilized activity after 5 regenerations. (C) 2012 Elsevier B.V. All rights reserved.
ISSN: 0376-7388
DOI: 10.1016/j.memsci.2012.01.022
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