Please use this identifier to cite or link to this item:
|標題:||Proton pumping inorganic pyrophosphatase of endoplasmic reticulum-enriched vesicles from etiolated mung bean seedlings||作者:||Kuo, S.Y.
|關鍵字:||endoplasmic reticulum;H+-pyrophosphatase;proton translocation;tonoplast;vacuole;vacuolar h+-pyrophosphatase;vigna-radiata l;submitochondrial;particles;membrane pyrophosphatase;tonoplast;plant;purification;atpases;localization;inhibition||Project:||Journal of Plant Physiology||期刊/報告no：:||Journal of Plant Physiology, Volume 162, Issue 2, Page(s) 129-138.||摘要:||
Endoplasmic reticulurn (ER)-enriched vesicles from etiolated hypocotyts of mung bean seedlings (Vigna radiata) were successfully isolated using Ficoll gradient and two-phase (polyethylene glycol-dextran) partition. The ER-enriched vesicles contained inorganic pyrophosphate (PPi) hydrolysis and its associated proton translocating activities. Antiserum prepared against vacuolar H+-pyrophosphatase (V-PPase, EC 22.214.171.124) did not inhibit this novel pyrophosphatase-dependent proton translocation, excluding the possible contamination of tonoptast vesicles in the ER-enriched membrane preparation. The optimal ratios of Mg2+/PPi (inorganic pyrophosphate) for enzymatic activity and PPj-dependent proton translocation of ER-enriched vesicles were, higher than those - of vacuolar membranes. The PPj-dependent Proton translocation of ER-enriched vesicles absolutely required the presence of monovalent cations with preference for K but could be inhibited by a common PPase inhibitor, F-. Furthermore, ER H+-pyrophosphatase exhibited some similarities and differences to vacuolar H+-PPases in cofactor/substrate ratios, pH profile, and concentration dependence of F-, imidodiphosphate (a PPj anatogue), and various chemical modifiers.
|Appears in Collections:||期刊論文|
Show full item record
TAIR Related Article
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.