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|標題:||Genome-Wide Transcript Expression Analysis in the Uterovaginal Junction in Association with Fertile Period in Tsaiya Ducks||作者:||Huang, H.L.
|關鍵字:||Differentially expressed transcripts;Duck;Fertile period;Uterovaginal;junction;oviductal sperm reservoir;female reproductive-tract;utero-vaginal;junction;in-vitro;storage tubules;hen oviduct;spermatozoa;epithelium;transport;binding||Project:||Journal of Reproduction and Development||期刊/報告no：:||Journal of Reproduction and Development, Volume 57, Issue 6, Page(s) 731-736.||摘要:||
We performed the first genome-wide expression analysis to compare the differences in gene expression in the female sperm reservoir of the duck reproductive tract between two groups with long and short fertile periods to identify factors that may be associated with the fertile period using an oligonucleotide microarray. RNA was extracted from the uterovaginal junction (UV junction) of the two groups. Affymetrix chips containing comprehensive coverage of 32773 transcripts were hybridized with biotin-labeled cRNA, and three biological repeats were performed. We identified 27 transcripts as being differentially regulated. Interestingly, by mapping the differentially expressed transcripts to annotated pathways, we found that Neuropeptide Y (NPY), the RNA expression of which was increased by 2.96-fold in the short-fertile-period group as compared with the long-fertile-period group in our experiment, has been shown to reduce blood flow and substance supply to local tissues. Enah/Vasp-like (EVL), the RNA expression of which was significantly increased by 1.77-fold in the short-fertile-period group as compared with the long-period group, has been demonstrated to be important in activated T-cells. In contrast, trafficking kinesin-binding protein 1 (TRAK1), the expression of which was increased by 2.33-fold in the long-period group as compared with its counterparts, has been suggested to inhibit precocious activation of sperm and prolong sperm life in the female sperm reservoir. The results of real-time PCR confirmed the data obtained by microarray analysis. Our study demonstrated that combining global gene expression investigation with annotated pathway resources contributes to the understanding of sperm life when sustained in the UV junction.
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