Complex formation between the lumenal domain of adenovirus E3-19k protein and the extracellular domain of class I MHC molecule in vitro

Abstract

The Ad2 E3-19k protein inhibits the transport of newly synthesized class I MHC molecules to the cell surface, thereby interfering with antigen presentation. The detai Is of the interaction between E3-19k protein and class I MHC molecules have not been well-defined. In this present study, we describe the use of gel filtration HPLC for confirming the binding interaction of two domain proteins, E3-19k and MHC class I antigen, and subsequently the characterization of protein complex by SDS-PAGE. Our results demonstrate the complex formation between Ad2 lumenal E3-19k (108 amino acids, wt 108) and HLA-A*0201 molecule in vitro. Titration experiments will be employed in the future to determine stoichiometry and verify the specific interactions.