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標題: Effect of oxidized regenerated cellulose on the healing of pharyngeal wound: An experimental animal study
作者: Liu, S.A.
Cheng, C.C.
Chen, J.S.
Hung, Y.W.
Chen, F.J.
Chiu, Y.T.
關鍵字: animal model;matrix metalloproteinases;oxidized regenerated cellulose;pharyngocutaneous fistula;wound healing;pharyngocutaneous fistula;extracellular-matrix;growth-factors;fibroblasts;repair;model;site
Project: Journal of the Chinese Medical Association
期刊/報告no:: Journal of the Chinese Medical Association, Volume 75, Issue 4, Page(s) 176-182.
Background: This study aimed to investigate the relationship between oxidized regenerated cellulose (ORC) and mucosa healing in an experimental animal model. Methods: Fifteen adult Sprague-Dawley rats were randomly divided into three groups that underwent different wound treatments. In Group 1, no pharyngeal wound was created. In Group 2, the pharyngeal wound was sutured with Prolene only. In Group 3, the pharyngeal wound was sutured with Prolene, and covered with one layer of ORC before closure of the skin wound. The animals were euthanized either 5 or 10 days after operation, and wound conditions were inspected and recorded. Specimens including sections of larynx and pharynx/upper esophagus were taken for microscopic and molecular biological examination. Results: The pharyngotomy/esophagotomy wounds achieved good healing outcomes 10 days after operation. Wounds treated with ORC had significantly diminished inflammatory cell infiltration in microscopic examination when compared with that of those without ORC 5 days after operation. The matrix metalloproteinases (MMP) expression level was higher in wounds of Group 2 and Group 3, when compared with that of group 1. In addition, the MMP expression level was lower in the ORC-treated wounds when compared with that of those without ORC. There was no significant difference in fibroblast proliferation, collagen deposition, endothelin-1, alpha-smooth muscle actin, and transforming growth factor beta 1 expression level between wounds treated with ORC and those without ORC. Conclusion: Reduced inflammatory response and decreased MMP expression level was observed in ORC-treated wounds. Whether ORC facilitates mucosa healing requires further investigation. Copyright (C) 2012 Elsevier Taiwan LLC and the Chinese Medical Association. All rights reserved.
ISSN: 1726-4901
DOI: 10.1016/j.jcma.2012.03.007
Appears in Collections:期刊論文

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