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|標題:||Isolation of a Xanthomonas campestris strain with elevated beta-galactosidase activity for direct use of lactose in xanthan gum production||作者:||Yang, T.C.
|關鍵字:||construction;whey||Project:||Letters in Applied Microbiology||期刊/報告no：:||Letters in Applied Microbiology, Volume 35, Issue 5, Page(s) 375-379.||摘要:||
Aims: To isolate a Xanthomonas campestris strain that can use lactose directly for xanthan gum production. Methods and Results: The presence of indigenous beta-galactosidase gene in the wild-type Xc17 was detected by PCR and Southern hybridization. Treatment of Xc17 with nitrous acid resulted in the isolation of Xc17L with a 3.5-fold elevation of beta-galactosidase activity capable of growing in lactose-based medium. Xc17L is stable for at least 100 generations in terms of beta-galactosidase expression. The amounts of xanthan produced by Xc17L in lactose-based medium are comparable to those in glucose-based medium. Conclusions: Xc17L is potentially useful for xanthan production from whey, a waste containing lactose. Significance and Impact of the Study: A lactose-utilizing strain of X. campestris strain can be constructed without incorporation of any exotic DNA or antibiotic resistance gene and therefore concern of a gene-modified organism and fear of a spread of an antibiotic-resistant gene are avoided.
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