Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/70732
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dc.contributor.authorHuang, E.J.en_US
dc.contributor.authorWu, C.C.en_US
dc.contributor.authorLee, S.D.en_US
dc.contributor.authorChen, J.H.en_US
dc.contributor.authorLiu, J.Y.en_US
dc.contributor.authorKo, J.L.en_US
dc.contributor.authorLin, J.A.en_US
dc.contributor.authorLu, M.C.en_US
dc.contributor.authorChen, L.M.en_US
dc.contributor.authorHuang, C.Y.en_US
dc.contributor.authorKuo, W.W.en_US
dc.date2006zh_TW
dc.date.accessioned2014-06-11T06:00:14Z-
dc.date.available2014-06-11T06:00:14Z-
dc.identifier.issn0300-8177zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/70732-
dc.description.abstractHepatocellular carcinoma (HCC), the major manifestation of primary liver cancer, is one of the most frequent and malignant cancers worldwide, especially in Taiwan. Estrogen receptors (ERs) have been reported to play either a proliferation- or apoptosis-enhancing role in the differentiation of cancers, including HCC. In a previous experiment, we showed that transient overexpressed estrogen receptor-alpha induced early stage HCC cell line Hep 3B cell apoptosis by increasing the hTNF-alpha gene expression in a ligand-independent manner. To further clarify if the apoptotic effect occurs in poorly differentiated HCC cell line, HA22T, and elucidate the roles of ERs and TNF-alpha, DNA fragmentation and caspase activity were measured in late stage HCC cell line, HA22T, by measuring the expression of hER-alpha and hER-beta using a Tetracycline-induciable system (Tet-on). Increased DNA fragmentation and caspase-3 activity were found in hER beta-overexpressed HA22T cells treated with estrogen (10(-8) M) but not in hER alpha-overexpressed HA22T cells. Using RT-PCR/PCR and western blotting in HA22T cells, overexpressed hER-beta was also found to increase the expression of hTNF-alpha mRNA and induce hTNF-alpha-dependent luciferase activity in a ligand-dependent manner. Additionally, LPS treatment and hER-beta overexpression both enhance caspase-8 activities, whereas neither hER-beta nor E-2 treatment affected caspase-9 activities. In addition, the overexpressed hER-beta plus E-2 enhanced DNA fragmentation and caspase-8 activities were only partially reduced by anti-hTNF-alpha (0.1ng/ml), which was possibly due to the involvement of P53 and TGF-beta. Taken together, our data indicates that overexpressed hER-beta but not hER-alpha may induce caspase-8-mediated apoptosis by increasing the hTNF-alpha gene expression in a ligand-dependent manner in poorly differentiated HA22T cells.en_US
dc.language.isoen_USzh_TW
dc.relationMolecular and Cellular Biochemistryen_US
dc.relation.ispartofseriesMolecular and Cellular Biochemistry, Volume 287, Issue 1-2, Page(s) 137-145.en_US
dc.relation.urihttp://dx.doi.org/10.1007/s11010-005-9092-4en_US
dc.subjectapoptotic effectsen_US
dc.subjectestrogen receptor-betaen_US
dc.subjectHA22T cellsen_US
dc.subjecttumor necrosisen_US
dc.subjectfactor-alphaen_US
dc.subjecthuman hepatoma-cellsen_US
dc.subjectbreast-cancer cellsen_US
dc.subjectmessenger-rnaen_US
dc.subjecter-betaen_US
dc.subjecthepatocellular-carcinomaen_US
dc.subjectcyclin d1en_US
dc.subjectchromosomal localizationen_US
dc.subjectmediateden_US
dc.subjectinhibitionen_US
dc.subjectprostate-canceren_US
dc.subjectactivationen_US
dc.titleOpposing action of estrogen receptors alpha and beta on tumor necrosis factor-alpha gene expression and caspase-8-mediated apoptotic effects in HA22T cellsen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1007/s11010-005-9092-4zh_TW
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en_US-
item.openairetypeJournal Article-
item.grantfulltextnone-
item.fulltextno fulltext-
item.cerifentitytypePublications-
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