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標題: Diverse Effects of beta-Carotene on Secretion and Expression of VEGF in Human Hepatocarcinoma and Prostate Tumor Cells
作者: Chen, H.Y.
Huang, S.M.
Yang, C.M.
Hu, M.L.
關鍵字: B16F10;beta-carotene;PC-3;SK-Hep-1;VEGF;delivery vehicle;supplementation;angiogenesis;stability;lycopene;mice
Project: Molecules
期刊/報告no:: Molecules, Volume 17, Issue 4, Page(s) 3981-3988.
Oral administration of beta-carotene (BC) was found to exert opposite effects on plasma levels of vascular endothelial growth factor (VEGF) in two animal models. One study in nude mice injected via tail vein with hepatocarcinoma SK-Hep-1 cells showed that BC decreases the plasma VEGF level, whereas the other study in nude mice injected subcutaneously with prostate tumor PC-3 cells showed that BC increases the plasma VEGF level. Herein we investigated whether BC (0.5-20 mu M) possesses diverse effects on VEGF secretion in SK-Hep-1, PC-3 and melanoma B16F10 cells. We found that incubation of SK-Hep-1 cells with BC (1-20 mu M) for 6 h significantly decreased VEGF secretion, whereas BC (1-10 mu M) significantly increased the VEGF secretion in PC-3 cells. However, these effects disappeared at 12 h of incubation. Similar effects occurred in VEGF mRNA and protein expression after treatment of SK-Hep-1 and PC-3 cells with BC for 6 h. In contrast, BC (0.5-20 mu M) did not affect mRNA and protein expression and secretion of VEGF in B16F10 cells. We also found that the proliferation of SK-Hep-1 and B16F10 cells was significantly inhibited by 20 mu M BC at 6 and 12 h of incubation, whereas the proliferation of PC-3 cells was significantly inhibited by 20 mu M BC at 12 h of incubation. In summary, the present study demonstrated the tumor-specific effect of BC on VEGF secretion in different cancer cell lines.
ISSN: 1420-3049
DOI: 10.3390/molecules17043981
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