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標題: Curcumin-Induced DNA Damage and Inhibited DNA Repair Genes Expressions in Mouse-Rat Hybrid Retina Ganglion Cells (N18)
作者: Lu, H.F.
Yang, J.S.
Lai, K.C.
Hsu, S.C.
Hsueh, S.C.
Chen, Y.L.
Chiang, J.H.
Lu, C.C.
Lo, C.
Yang, M.D.
Chung, J.G.
關鍵字: Curcumin;DNA damage;DNA repair;N18 mouse-rat hybrid retina ganglion;cells;human breast-cancer;induced apoptosis;gel-electrophoresis;lipid-peroxidation;alzheimers-disease;crude extracts;pathway;nuclear;mitochondrial;cytotoxicity
Project: Neurochemical Research
期刊/報告no:: Neurochemical Research, Volume 34, Issue 8, Page(s) 1491-1497.
Curcumin is reported to be a potent inhibitor of the initiation and promotion of many cancer cells. We investigated to examine whether or not curcumin induce DNA damage in mouse-rat hybrid retina ganglion cell line N18 cells. The Comet assay showed that incubation of N18 cells with 10, 25 and 30 mu M of curcumin led to a longer DNA migration smear (Comet tail). The DNA gel electrophoresis showed that 20 mu M of curcumin for 24 and 48 h treatment induced DNA damage and fragments in N18 cells. The real time PCR analysis showed that 20 mu M of curcumin for 48 h treatment decreased ATM, ATR, BRCA1, 14-3-3 sigma, DNA-PK and MGMT mRNA, and ATM and MGMT mRNA expression were inhibited in a time-dependent manner. Our results indicate that curcumin caused DNA damage and inhibited DNA repair genes which may be the factors for curcumin-inhibited cell growth.
ISSN: 0364-3190
DOI: 10.1007/s11064-009-9936-5
Appears in Collections:期刊論文

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