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標題: Caspase-14 is an anti-apoptotic protein targeting apoptosis-inducing factor in lung adenocarcinomas
作者: Fang, H.Y.
Chen, C.Y.
Hung, M.F.
Hsiao, Y.T.
Chiang, T.C.
Lin, T.Y.
Chang, H.W.
Chow, K.C.
Ko, W.J.
關鍵字: caspase-14;apoptosis-inducing factor;cisplatin resistance;lung;adenocarcinoma;anti-apoptosis;independent cell-death;dna-damage response;epidermal differentiation;cancer cells;expression;activation;cleavage;survival;smoking;p53
Project: Oncology Reports
期刊/報告no:: Oncology Reports, Volume 26, Issue 2, Page(s) 359-369.
Using apoptosis-inducing factor protein as bait in a yeast hybrid assay to screen protein libraries, we identified three proteins that interacted with apoptosis-inducing factor: human homolog of yeast Rad23 protein A (hHR23A), microsomal glutathione S-transferase and caspase-14 (casp-14). In this study, we investigated the expression and function of casp-14 in lung adenocarcinomas (LADC). Our results showed that monoclonal antibodies were specific to casp-14, and that casp-14 was highly expressed in LADC. Casp-14 overexpression correlated with tumor stage, cell differentiation and lymphovascular involvement, suggesting that casp-14 was associated with tumor cell growth and metastatic potential. In vitro, casp-14 interacted with apoptosis-inducing factor, and silencing of casp-14 expression reduced cisplatin resistance. Our data suggest that casp-14 is an anti-apoptotic protein targeting apoptosis-inducing factor and increases cisplatin resistance in LADC cells.
ISSN: 1021-335X
DOI: 10.3892/or.2011.1292
Appears in Collections:期刊論文

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