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標題: Houttuynia cordata Thunb extract induces apoptosis through mitochondrial-dependent pathway in HT-29 human colon adenocarcinoma cells
作者: Tang, Y.J.
Yang, J.S.
Lin, C.F.
Shyu, W.C.
Tsuzuki, M.
Lu, C.C.
Chen, Y.F.
Lai, K.C.
關鍵字: Houttuynia cordata Thunb;human colon adenocarcinoma cell line HT-29;apoptosis;mitochondrial-dependent pathway;bidens-pilosa;cancer;chemoprevention;inhibitors;induction;selenium;stress;parp-1;virus;model
Project: Oncology Reports
期刊/報告no:: Oncology Reports, Volume 22, Issue 5, Page(s) 1051-1056.
The Houttuynia cordata Thunb (HCT) extract has been used as a traditional Chinese herb medicine and as well as an effective drug for treating allergic inflammation for thousands of years. In this study, we investigated the anticancer activity of HCT and its molecular mechanisms in the human colon adenocarcinoma cell line HT-29. HCT inhibited HT-29 cell viability in a dose- and time-dependent manner by MTT assay. Treatment with 450 mu g/ml of HCT for 48 and 72 h led to DNA damage and apoptosis by DAPI staining and comet assay. HCT increased reactive oxygen species production and decreased the levels of mitochondria membrane potential (MMP) in HT-29 cells by flow cytometry analysis. HCT caused the release of cytochrome c, Apaf-1, pro-caspase-9 and AIF from mitochondria via a decrease of the MMP. The decrease of MMP was then associated with a decrease in the ratio of Bax/Bcl-2 and activation of caspase-9 and -3 by Western blotting and caspase activity assay. Caspase-9 and -3 inhibitors almost completely suppressed HCT-induced caspase-9 and -3 activities. Our results demonstrated that the HCT-induced apoptosis in human colon adeno-carcinoma cell line HT-29 might be related to a mitochondrial- dependent pathway.
ISSN: 1021-335X
DOI: 10.3892/or_00000535
Appears in Collections:期刊論文

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