Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/71014
DC FieldValueLanguage
dc.contributor.authorHsu, H.T.en_US
dc.contributor.authorBarzuna, L.en_US
dc.contributor.authorHsu, Y.H.en_US
dc.contributor.authorBliss, W.en_US
dc.contributor.authorPerry, K.L.en_US
dc.date2000zh_TW
dc.date.accessioned2014-06-11T06:00:42Z-
dc.date.available2014-06-11T06:00:42Z-
dc.identifier.issn0031-949Xzh_TW
dc.identifier.urihttp://hdl.handle.net/11455/71014-
dc.description.abstractUsing a mixture of isolates of Cucumber mosaic virus (CMV) from subgroups I and II as immunogens, 20 mouse hybridoma cell lines secreting monoclonal antibodies were produced. A reliable method for efficient detection and accurate subgrouping of CMV isolates has been developed. Tests with 12 well-characterized strains of CMV and other cucumoviruses demonstrated the presence of epitopes that were virus and subgroup specific. Analyses of 109 accessions of CMV isolates collected from various parts of the world revealed 70% were subgroup I, with 20% identified as subgroup II. Seven isolates (6%) did not react with group-specific antibodies but did react with antibodies that recognized all CMV isolates. Differential reactions among isolates suggested a total of 10 epitopes were recognized. The antigenic diversity among subgroup II CMVs was greater than for the subgroup I isolates, even though fewer subgroup II isolates were tested.en_US
dc.language.isoen_USzh_TW
dc.relationPhytopathologyen_US
dc.relation.ispartofseriesPhytopathology, Volume 90, Issue 6, Page(s) 615-620.en_US
dc.relation.urihttp://dx.doi.org/10.1094/phyto.2000.90.6.615en_US
dc.subjectwilt virusen_US
dc.subjectrnaen_US
dc.subjecthybridizationen_US
dc.subjectstrainsen_US
dc.subjectcmven_US
dc.titleIdentification and subgrouping of Cucumber mosaic virus with mouse monoclonal antibodiesen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1094/phyto.2000.90.6.615zh_TW
item.languageiso639-1en_US-
item.openairetypeJournal Article-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.fulltextno fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
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