Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/71031
DC FieldValueLanguage
dc.contributor.authorChiu, C.C.en_US
dc.contributor.authorLin, C.S.en_US
dc.contributor.authorHsia, A.P.en_US
dc.contributor.authorSu, R.C.en_US
dc.contributor.authorLin, H.L.en_US
dc.contributor.authorTsay, Y.F.en_US
dc.date2004zh_TW
dc.date.accessioned2014-06-11T06:00:45Z-
dc.date.available2014-06-11T06:00:45Z-
dc.identifier.issn0032-0781zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/71031-
dc.description.abstractUnlike nitrate uptake of plant roots, less is known at the molecular level about how nitrate is distributed in various plant tissues. In the present study, characterization of the nitrate transporter, AtNRT1:4, revealed a special role of petiole in nitrate homeostasis. Electrophysiological studies using Xenopus oocytes showed that AtNRT1:4 was a low-affinity nitrate transporter. Whole-mount in situ hybridization and RT-PCR demonstrated that AtNRT1:4 was expressed in the leaf petiole. In the wild type, the leaf petiole had low nitrate reductase activity, but a high nitrate content, indicating that it is the storage site for nitrate, whereas, in the atnrt1:4 mutant, the petiole nitrate content was reduced to 50-64% of the wild-type level. Moreover, atnrt1:4 mutant leaves were wider than wild-type leaves. This study revealed a critical role of AtNRT1:4 in regulating leaf nitrate homeostasis, and the deficiency of AtNRT1: 4 can alter leaf development.en_US
dc.language.isoen_USzh_TW
dc.relationPlant and Cell Physiologyen_US
dc.relation.ispartofseriesPlant and Cell Physiology, Volume 45, Issue 9, Page(s) 1139-1148.en_US
dc.relation.urihttp://dx.doi.org/10.1093/pcp/pch143en_US
dc.subjectArabidopsisen_US
dc.subjectleaf developmenten_US
dc.subjectnitrateen_US
dc.subjectNRT1en_US
dc.subjectpetioleen_US
dc.subjectdetermining nitrogen needsen_US
dc.subjectfunctional-characterizationen_US
dc.subjectarabidopsis-thalianaen_US
dc.subjectpeptide transportersen_US
dc.subjectt-dnaen_US
dc.subjectgeneen_US
dc.subjectchl1en_US
dc.subjectcloningen_US
dc.subjectfamilyen_US
dc.subjectexpressionen_US
dc.titleMutation of a nitrate transporter, AtNRT1 : 4, results in a reduced petiole nitrate content and altered leaf developmenten_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1093/pcp/pch143zh_TW
item.cerifentitytypePublications-
item.grantfulltextnone-
item.languageiso639-1en_US-
item.fulltextno fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
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