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標題: Measurement of gas exchange rates in plant tissue culture vessels
作者: Chen, C.C.
Chen, J.N.J.
關鍵字: gas exchange rate;model;tissue culture;vessels;growth
Project: Plant Cell Tissue and Organ Culture
期刊/報告no:: Plant Cell Tissue and Organ Culture, Volume 71, Issue 2, Page(s) 103-109.
The aerial microenvironment in culture vessels has a significant effect on the growth and development of plantlets in vitro. Since the gas exchange between outside air and inner air can influence the microenvironment of culture vessel, it is necessary to measure the air exchange rate for various vessels. In this study, water vapor was used as the tracer gas, and the change of absolute humidity inside the vessel was calculated continuously by the measured values of a relative humidity sensing element. The outside environment was maintained at constant humidity level by a saturated salt solution. The RH data were transformed into absolute humidity and the specific humidity ratio. The air exchange rates of several tissue culture vessels were then calculated. The exchange rate was between 0.0145 h(-1) to 0.0376 h(-1). This technique provides an inexpensive, rapid and simple way to determine the air exchange rate of a culture vessel within a short period. The effects of the air current velocities on the exchange rates of vessels were also determined.
ISSN: 0167-6857
DOI: 10.1023/a:1019909520132
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