Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/71071
DC FieldValueLanguage
dc.contributor.authorLin, S.S.en_US
dc.contributor.authorMartin, R.en_US
dc.contributor.authorMongrand, S.en_US
dc.contributor.authorVandenabeele, S.en_US
dc.contributor.authorChen, K.C.en_US
dc.contributor.authorJang, I.C.en_US
dc.contributor.authorChua, N.H.en_US
dc.date2008zh_TW
dc.date.accessioned2014-06-11T06:00:49Z-
dc.date.available2014-06-11T06:00:49Z-
dc.identifier.issn0960-7412zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/71071-
dc.description.abstractUbiquitination plays important roles in plant development, including programmed cell death. Here, we characterize a novel membrane-bound RING motif protein, encoded by RING1, that is expressed at a low level in all Arabidopsis tissues but can be upregulated by fumonisin B1 (FB1) treatment and pathogen infection. RING1 displays E3 ubiquitin ligase activity in vitro, which is dependent on the integrity of the RING motif. GFP fusion protein localization and cell fractionation experiments show that this E3 ligase is associated with the lipid rafts of plasma membranes. Knock-down of RING1 transcripts using artificial microRNA (amiR-R1(159)) leads to FB1 hyposensitivity, but overexpression of RING1 confers hypersensitivity. Additionally, expression of the pathogenesis-related 1 (PR-1) gene is lower and delayed in amiR-R1(159) plants compared with wild-type and RING1-overexpressing plants. The FB1 hyposensitivity of amiR-R1(159) plants can be rescued by expression of cleavage-resistant RING1mut transcripts. Our results suggest that RING1 acts as a signal from the plasma membrane lipid rafts to trigger the FB1-induced plant programmed cell death pathway.en_US
dc.language.isoen_USzh_TW
dc.relationPlant Journalen_US
dc.relation.ispartofseriesPlant Journal, Volume 56, Issue 4, Page(s) 550-561.en_US
dc.relation.urihttp://dx.doi.org/10.1111/j.1365-313X.2008.03625.xen_US
dc.subjectprogrammed cell deathen_US
dc.subjectubiquitinationen_US
dc.subjectRING motifen_US
dc.subjectlipid raften_US
dc.subjectfumonisinen_US
dc.subjectB1en_US
dc.subjectubiquitin-proteasome pathwayen_US
dc.subjectbax inhibitor-1en_US
dc.subjecthypersensitive responseen_US
dc.subjectprotooncogene bcl-2en_US
dc.subjectplant-cellsen_US
dc.subjectapoptosisen_US
dc.subjectexpressionen_US
dc.subjectthalianaen_US
dc.subjectceramideen_US
dc.subjectyeasten_US
dc.titleRING1 E3 ligase localizes to plasma membrane lipid rafts to trigger FB1-induced programmed cell death in Arabidopsisen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1111/j.1365-313X.2008.03625.xzh_TW
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextno fulltext-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.languageiso639-1en_US-
item.openairetypeJournal Article-
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