Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/71076
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dc.contributor.authorKanjanaphachoat, P.en_US
dc.contributor.authorWei, B.Y.en_US
dc.contributor.authorLo, S.F.en_US
dc.contributor.authorWang, I.W.en_US
dc.contributor.authorWang, C.S.en_US
dc.contributor.authorYu, S.M.en_US
dc.contributor.authorYen, M.L.en_US
dc.contributor.authorChiu, S.H.en_US
dc.contributor.authorLai, C.C.en_US
dc.contributor.authorChen, L.J.en_US
dc.date2012zh_TW
dc.date.accessioned2014-06-11T06:00:49Z-
dc.date.available2014-06-11T06:00:49Z-
dc.identifier.issn0167-4412zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/71076-
dc.description.abstractA mutant M47286 with a stunted growth, low fertility and dark-brown phenotype was identified from a T-DNA-tagged rice mutant library. This mutant contained a copy of the T-DNA tag inserted at the location where the expression of two putative tryptophan decarboxlyase genes, TDC-1 and TDC-3, were activated. Enzymatic assays of both recombinant proteins showed tryptophan decarboxlyase activities that converted tryptophan to tryptamine, which could be converted to serotonin by a constitutively expressed tryptamine 5' hydroxylase (T5H) in rice plants. Over-expression of TDC-1 and TDC-3 in transgenic rice recapitulated the stunted growth, dark-brown phenotype and resulted in a low fertility similar to M47286. The degree of stunted growth and dark-brown color was proportional to the expression levels of TDC-1 and TDC-3. The levels of tryptamine and serotonin accumulation in these transgenic rice lines were also directly correlated with the expression levels of TDC-1 and TDC-3. A mass spectrometry assay demonstrated that the dark-brown leaves and hulls in the TDC-overexpressing transgenic rice were caused by the accumulation of serotonin dimer and that the stunted growth and low fertility were also caused by the accumulation of serotonin and serotonin dimer, but not tryptamine. These results represent the first evidence that over-expression of TDC results in stunted growth, low fertility and the accumulation of serotonin, which when converted to serotonin dimer, leads to a dark brown plant color.en_US
dc.language.isoen_USzh_TW
dc.relationPlant Molecular Biologyen_US
dc.relation.ispartofseriesPlant Molecular Biology, Volume 78, Issue 6, Page(s) 525-543.en_US
dc.relation.urihttp://dx.doi.org/10.1007/s11103-012-9882-5en_US
dc.subjectTryptophan decarboxylaseen_US
dc.subjectTryptophanen_US
dc.subjectTryptamineen_US
dc.subjectSerotonin dimeren_US
dc.subjectBrownen_US
dc.subjectpigmentationen_US
dc.subjectT-DNA taggingen_US
dc.subjectTransgenic riceen_US
dc.subjecttyrosine decarboxylaseen_US
dc.subjectbiosynthesisen_US
dc.subjecttryptamineen_US
dc.subjectplantsen_US
dc.subjecttransformationen_US
dc.subjectoxidationen_US
dc.subjecttyramineen_US
dc.subjectgeneen_US
dc.subjectpathwayen_US
dc.subjectleadsen_US
dc.titleSerotonin accumulation in transgenic rice by over-expressing tryptophan decarboxlyase results in a dark brown phenotype and stunted growthen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1007/s11103-012-9882-5zh_TW
item.cerifentitytypePublications-
item.grantfulltextnone-
item.languageiso639-1en_US-
item.fulltextno fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
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