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標題: Abundantly expressed hepatic genes and their differential expression in liver of prelaying and laying geese
作者: Yen, C.F.
Lin, E.C.
Wang, Y.H.
Wang, P.H.
Lin, H.W.
Hsu, J.C.
Wu, L.S.
Jiang, Y.N.
Ding, S.T.
關鍵字: very low density apolipoprotein II;laying geese;liver;ovotransferrin;ovoinhibitor;acid-binding-protein;early posthatch development;yolk-sac membrane;meleagridis-gallopavo;docosahexaenoic acid;gallus-domesticus;lipid-metabolism;sequence;estrogen;evolution
Project: Poultry Science
期刊/報告no:: Poultry Science, Volume 88, Issue 9, Page(s) 1955-1962.
Geese have a short egg-laying period and a low egg production rate. To induce and maintain egg laying, genes related to generating hepatic lipid for yolk deposition should be adequately expressed. Liver mRNA from 6 laying geese was extracted and used for construction of a full-length enriched cDNA library. About 2,400 clones containing gene sequences were determined and National Center for Biotechnology Information Gallus gallus Gene Index databases were used to compare and analyze these sequences. Ten highly expressed genes were selected to determine the differential expression between laying and prelay goose liver. Tissue distribution data showed that very low density apolipoprotein II, liver type fatty acid binding protein, vitellogenin I, and vitellogenin II transcripts were specifically expressed in the liver of laying geese. Ovoinhibitor, preproalbumin, alpha-2-hs-glycoprotein, and vitamin D binding protein mRNA were highly expressed in the liver and to a lesser extent in other tissues. Ovotransferrin mRNA was expressed in liver, ovary, oviduct, shell gland, brain, and adipose tissues. The concentration of transthyretin mRNA was high in the liver and brain. The mRNA concentrations of liver type fatty acid binding protein, alpha-2-hs-glycoprotein, and transthyretin in the livers of laying and prelay geese were not different. The concentrations of hepatic ovotransferrin, ovoinhibitor, preproalbumin, very low density apolipoprotein II, vitellogenin I, vitellogenin II, and vitamin D binding protein mRNA were higher in the liver of laying geese than in prelay geese, suggesting that these genes may be involved in laying function or lipid metabolism related to egg formation.
ISSN: 0032-5791
DOI: 10.3382/ps.2008-00473
Appears in Collections:期刊論文

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