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|標題:||Qualitative and comparative proteomic analysis of Xanthomonas campestris pv. campestris 17||作者:||Chung, W.J.
|關鍵字:||plant pathogen;Xanthomonas campestris pv. campestris;escherichia-coli;trigger factor;protein;pathogenicity;expression;genes;cell;biosynthesis;vesicatoria;degradation||Project:||Proteomics||期刊/報告no：:||Proteomics, Volume 7, Issue 12, Page(s) 2047-2058.||摘要:||
The bacterium Xanthomonas campestris pathovar campestris (XCC) 17 is a local isolate that causes crucifer black rot disease in Taiwan. In this study, its proteome was separated using 2-DE and the well-resolved proteins were excised, trypsin digested, and analyzed by MS. Over 400 protein spots were analyzed and 281 proteins were identified by searching the MS or MS/MS spectra against the proteome database of the closely related XCC ATCC 33913. Functional categorization of the identified proteins matched 141 (50%) proteins to 81 metabolic pathways in the kyoto Encyclopedia of Genes and Genomes (KEGG) database. In addition, we performed a comparative proteome analysis of the pathogenic strain 17 and an avirulent strain 11A to reveal the virulence-related proteins. We detected 22 up-regulated proteins in strain 17 including the degrading enzymes EngXCA, HtrA, and PepA, which had been shown to have a role in pathogenesis in other bacteria, and an anti-host defense protein, Ohr. Thus, further functional studies of these up-regulated proteins with respect to their roles in XCC pathogenicity are suggested.
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