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|標題:||An enzyme-linked immunosorbent assay for the detection of antibody to avian reovirus by using protein sigma B as the coating antigen||作者:||Shien, J.H.
|關鍵字:||monoclonal-antibodies;strain s1133;serum;elisa||Project:||Research in Veterinary Science||期刊/報告no：:||Research in Veterinary Science, Volume 69, Issue 2, Page(s) 107-112.||摘要:||
An enzyme-linked immunosorbent assay using the expressed protein sigma B as the coating antigen (sigma B-ELISA) for detecting antibody to avian reovirus (ARV) in chickens was developed and compared with a conventional ELISA. Both ELISAS and a serum neutralisation (SN) test were used to test the sera from experimentally vaccinated and farm chickens. The sigma B-ELISA could clearly distinguish the sn-positive and -negative sera in 38-week-old chickens. The correlation rate between SN and a sigma B-ELISA was 100 per cent (65/65), and that between SN and conventional ELISA was 84 per cent (55/65). With the sigma B-ELISA, all SN-negative sera had low absorbance values (below 0.06), and the absorbance values correlated closely with the SN titres. However, the sera which were antibody-negative by SN had various absorbance values, ranging from 0.07 to 0.39 in the conventional ELISA. Hence, the sigma B-ELISA had lower nonspecific binding reactions than the conventional ELISA against sera from ARV-negative birds. Antibody against ARV could be detected by a B-ELISA after vaccination. Absorbance values peaked 4 weeks after vaccination at 2 weeks of age and were maintained until the birds were 27 weeks old. The results suggest that the presence of antibody against viral protein sigma B in birds may be used as a good indicator by the sigma B-ELISA for detecting immune status of a chicken flock or to detect chickens infected with ARV. (C) 1000 Harcourt Publishers Ltd.
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