Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/71223
標題: Experimental infections of rabbits with rabbit haemorrhagic disease virus monitored by polymerase chain reaction
作者: Shien, J.H.
Shieh, H.K.
Lee, L.H.
關鍵字: hare syndrome viruses;capsid protein gene;hemorrhagic-disease;calicivirus;sequences;diagnosis
Project: Research in Veterinary Science
期刊/報告no:: Research in Veterinary Science, Volume 68, Issue 3, Page(s) 255-259.
摘要: 
Adult and 4-5-week-old rabbits were inoculated subcutaneously with rabbit haemorrhagic disease virus (RHDV). Samples were prepared from various tissues at intervals postinoculation (PI) for the detection of viral RNA and antigens. Using a haemagglutination test (HAT), viral antigens were detected in the liver, bile and spleen of the adult rabbits at and after 36 h PI. The reverse transcription-polymerase chain reaction (RT-PCR) Showed that RHDV RNA was present in the liver, bile and spleen as early as Is hours Pr. whereas lung, kidney, thymus, mesenteric lymph node and buffy coat were found to be positive after more than 26 hours pi. In addition, viral RNA in urine and faeces showed a variable positivity at and after 36 hours pr. In the young rabbits, RT-PCR showed that RHDV RNA was present as early as 1 day PI in the liver, bile, spleen and buffy coat; whereas lung, kidney, thymus? mesenteric lymph node and faeces were found to be positive at and after 2 days pr. Bile and spleen were the only samples in which viral RNA was detected throughout the length of the experiment. Virus was not reactivated in six recovered virus-inoculated rabbits treated with dexamethasone or a classical swine fever virus vaccine. Using a haemagglutination inhibition test and an ELISA, antibody titres increased rapidly from one week PI onwards, peaked at approximately three weeks of age, and were maintained throughout the length of the experiment.
URI: http://hdl.handle.net/11455/71223
ISSN: 0034-5288
DOI: 10.1053/rvsc.1999.0372
Appears in Collections:期刊論文

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