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標題: Impedimetric monitoring of cell attachment on interdigitated microelectrodes
作者: Chang, B.W.
Chen, C.H.
Ding, S.J.
Chen, D.C.H.
Chang, H.C.
關鍵字: cell attachment;RGD-C peptide;interdigitated microelectrode;impedance;analysis;impedance analysis;tissue-culture;extracellular-matrix;substrate;surfaces;fibronectin;adhesion;behavior;fibroblasts;micromotion
Project: Sensors and Actuators B-Chemical
期刊/報告no:: Sensors and Actuators B-Chemical, Volume 105, Issue 2, Page(s) 159-163.
An on-line and real-time impedimetric system was developed to monitor and quantify cell attachment on interdigitated microetectrodes. Changes in admittance at frequency lower than 10 kHz were positively con-elated with the extent of cell attachment (10(2) to 10(7) cells/cm(2)). As udaed from the admittance change, the onset and the extent of cell attachment were facilitated when the electrodes were modified with RGD-C peptide, an artificial peptide with a high affinity toward cellulose surface. The system is useful in estimating seeding cell density and characterizing bio-compatible materials with suitable cell affinity. (c) 2004 Elsevier B.V. All rights reserved.
ISSN: 0925-4005
DOI: 10.1016/j.snb.2004.05.048
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