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標題: Community structure and functional diversity of indigenous fluorescent Pseudomonas of long-term swine compost applied maize rhizosphere
作者: Chang, C.Y.
Chao, C.C.
Chao, W.L.
關鍵字: rhizosphere;compost;fluorescent Pseudomonas;16S RFLP;catabolic;functions;l-rhamnose;microbial communities;escherichia-coli;organic-matter;sewage-sludge;plant-growth;soil;metabolism;bacteria;stenotrophomonas
Project: Soil Biology & Biochemistry
期刊/報告no:: Soil Biology & Biochemistry, Volume 40, Issue 2, Page(s) 495-504.
The community structure and catabolic functional diversity of indigenous rhizosphere fluorescent pseudomonad isolates recovered from un-amended soil (control treated) and following long-term (6 years) swine compost application (compost treated) were studied. Soils collected for the pot experiments were from a Taiwanese experimental farm established in 1995 to study the long-term effects of crop rotation and organic amendment. In the pot experiments, corn seed was cultured; after the elongation stage, 13 and 32 indigenous rhizosphere fluorescent pseudomonad isolates were collected from compost-treated (n = 13) and control-treated (17 = 32) soils. All isolates were classified by 16S rDNA restriction fragment length polymorphism (RFLP) and unweighted pair-group method using arithmetic averages analyses. Most (84.4%) of the control-treated soil isolates belonged to genotype A, which corresponded to Pseudomonas putida based on 16S rDNA sequences analysis. However, only 61.5% of compost-treated soil isolates were from genotype A; 30.7% of the isolates belonged to genotype B, whose 16S rDNA profile corresponded to Stenotrophomonas maltophilia. Differing catabolic potentials of the P. putida isolates from control- and compost-treated soils were evident; isolates from control-treated soils utilized organic acids including citric acid, cis-aconitic acid, and formic acid more actively than compost-treated soil isolates. Moreover, the latter isolates more efficiently utilized carbohydrates of i-erythritol and L-rhamnose than P. putida isolates from control-treated soil, consistent with the alteration in the catabolic functions of indigenous rhizosphere P. putida by long-term compost application. (c) 2007 Elsevier Ltd. All rights reserved.
ISSN: 0038-0717
DOI: 10.1016/j.soilbio.2007.09.014
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