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標題: Isolation of a recombinant Bacillus sp TS-23 alpha-amylase by adsorption-elution on raw starch
作者: Lin, L.L.
Lo, H.F.
Chen, J.N.
Ku, K.L.
Hsu, W.H.
關鍵字: adsorption-elution;alpha-amylase;Bacillus sp TS-23;raw starch;escherichia-coli;2 forms;glucoamylase;purification;expression;digestion;granules;behavior;gene
Project: Starch-Starke
期刊/報告no:: Starch-Starke, Volume 54, Issue 8, Page(s) 338-342.
A Bacillus sp. TS-23 alpha-amylase produced by recombinant Escherichia coli was adsorbed onto raw starch and the adsorbed enzyme was eluted with maltose or maltodextrin in 50 mM Tris/HCl buffer (pH 8.5). The adsorption-elution procedure resulted in a yield of 53% alpha-amylase activity and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS/PAGE) analysis showed that the eluted alpha-amylase had a molecular mass of approximately 64 kDa. Raw starch could be used repeatedly in the adsorption-elution cycle with good reproducibility. Scanning electron microscopy of the isolated corn starch exhibited a smooth appearance of the granules before adsorption and only a small change in appearance after three adsorption-elution cycles. These results suggest that the raw starch adsorption-elution technique has a great potential in the isolation of Bacillus sp. TS-23 alpha-amylase from the culture broth of recombinant E coli.
ISSN: 0038-9056
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