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|標題:||Isolation of a recombinant Bacillus sp TS-23 alpha-amylase by adsorption-elution on raw starch||作者:||Lin, L.L.
|關鍵字:||adsorption-elution;alpha-amylase;Bacillus sp TS-23;raw starch;escherichia-coli;2 forms;glucoamylase;purification;expression;digestion;granules;behavior;gene||Project:||Starch-Starke||期刊/報告no：:||Starch-Starke, Volume 54, Issue 8, Page(s) 338-342.||摘要:||
A Bacillus sp. TS-23 alpha-amylase produced by recombinant Escherichia coli was adsorbed onto raw starch and the adsorbed enzyme was eluted with maltose or maltodextrin in 50 mM Tris/HCl buffer (pH 8.5). The adsorption-elution procedure resulted in a yield of 53% alpha-amylase activity and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS/PAGE) analysis showed that the eluted alpha-amylase had a molecular mass of approximately 64 kDa. Raw starch could be used repeatedly in the adsorption-elution cycle with good reproducibility. Scanning electron microscopy of the isolated corn starch exhibited a smooth appearance of the granules before adsorption and only a small change in appearance after three adsorption-elution cycles. These results suggest that the raw starch adsorption-elution technique has a great potential in the isolation of Bacillus sp. TS-23 alpha-amylase from the culture broth of recombinant E coli.
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