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標題: Rapid identification of Lactobacillus plantarum group using the SNaPshot minisequencing assay
作者: Huang, C.H.
Chang, M.T.
Huang, M.C.
Lee, F.L.
關鍵字: Species identification;Single nucleotide polymorphisms;SNaPshot;minisequencing;Lactobacillus plantarum group;species level;strains;pcr;differentiation;microorganisms;fermentations;diversity;nutrition;products
Project: Systematic and Applied Microbiology
期刊/報告no:: Systematic and Applied Microbiology, Volume 34, Issue 8, Page(s) 586-589.
This study used SNaPshot minisequencing for species identification within the Lactobacillus plantarum group. A SNaPshot minisequencing assay using dnaK as a target gene was developed, and five SNP primers were designed by analysing the conserved regions of the dnaK sequences. The specificity of the minisequencing assay was evaluated using 35 strains oft. plantarum group species. The results showed that the SNaPshot minisequencing assay was able to unambiguously and simultaneously discriminate strains belonging to the species L. plantarum subsp. plantarum, L. plantarum subsp. argentoratensis. Lactobacillus paraplantarum, Lactobacillus pentosus and Lactobacillus fabifermentans. In conclusion, a rapid, accurate and cost-effective assay was successfully developed for species identification of the members of the L. plantarum group. (C) 2011 Elsevier GmbH. All rights reserved.
ISSN: 0723-2020
DOI: 10.1016/j.syapm.2011.02.006
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