Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/71522
標題: Effect of diallyl sulfide and diallyl disulfide, the active principles of garlic, on the aflatoxin B-1-induced DNA damage in primary rat hepatocytes
作者: Sheen, L.Y.
Wu, C.C.
Lii, C.K.
Tsai, S.J.
關鍵字: diallyl sulfide;diallyl disulfide;garlic;primary rat hepatocytes;aflatoxin B-1;n-acetyltransferase activity;glutathione s-transferases;differential;induction;organosulfur compounds;allyl sulfides;cell viability;tumor;cells;liver;carcinogenesis;mice
Project: Toxicology Letters
期刊/報告no:: Toxicology Letters, Volume 122, Issue 1, Page(s) 45-52.
摘要: 
The objective of this study was to investigate the effect of the active principles in garlic-diallyl sulfide (DAS) and diallyl disulfide (DADS)-on aflatoxin B-1 (AFB(1))-induced DNA damage in primary rat hepatocytes. Primary rat hepatocytes, induced with DNA damage using 10 muM AFB(1) were used as an experimental model. According to the results of LDH leakage, 0.5 and 2 mM of DAS or 0.5 and 1 mM of DADS significantly increased the viability of hepatocytes compared with the AFB(1) controls after 4, 8 and 24 h treatment (P <0.05). According to the results of unscheduled DNA synthesis (UDS) test. 0.5 and 2 mM of DAS or 0.5 acid 1 mM of DADS could significantly decrease the DNA damage induced by AFB(1) (P<0.05). Furthermore, 0.5 and 2 mM DAS or 0.5 and 1 mM DADS could increase the glutathione S-transferase (GST) and glutathione peroxidase (GPx) activities as compared with the AFB(1) controls after 24 h treatment (P<0.05). Results of immunoblot analysis of cytosolic GST isoenzyme indicate that the levels of GST isoform Ya, Yb2 and Yc were markly increased after treatment with 0.5 and 2 mM DAS or 0.5 and 1 mM DADS compared with the AFB(1) control. These results indicate that 0.5 and 2 mM DAS or 0.5 and 1 mM DADS might protect hepatocytes from AFB(1)-induced DNA damage via increasing the activities of GST and GPx. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.
URI: http://hdl.handle.net/11455/71522
ISSN: 0378-4274
DOI: 10.1016/s0378-4274(01)00347-2
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