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標題: Phenotypic changes in the Japanese encephalitis virus after one passage in Neuro-2a cells: Generation of attenuated strains of the virus
作者: Chiou, S.S.
Chen, W.J.
關鍵字: live-attenuated vaccine;Japanese encephalitis virus;virus selection;west-nile-virus;envelope protein;heparan-sulfate;unconventional;ecosystem;membrane-fusion;in-vivo;vaccine;mice;neurovirulence;adaptation
Project: Vaccine
期刊/報告no:: Vaccine, Volume 26, Issue 1, Page(s) 15-23.
Live attenuated strains of the Japanese encephalitis (JE) virus are known to form in various cultured cells. In this study, we selected attenuated JE virus variants by passing the parent virus strains once through Neuro-2a cells, showing that the selection intensity ranged 2-1000-fold after infection for 3 d. The selection of attenuated variants appeared in association with mutations on the envelope (E) glycoprotein. This is likely determined by the differential binding abilities of specific E proteins with highly sulfated glycosaminoglycans on the cell. surface. A plaque-purification method showed that Neuro-2a-selected variants were usually less neurovirulent, Leading to a longer survival time of intracerebrally inoculated mice. Specific selected variants (mostly with the small-plaque phenotype) were shown to be more efficient at replication in Vero cells and less virulent, particularly those with substitution of the E-138 (Glu -> Lys) residue. As a result, most, if not all, Low-virulent variants were generated in a relatively short time, all. which have the potential to be live-attenuated vaccine candidates of the JE virus. (c) 2007 Elsevier Ltd. All rights reserved.
ISSN: 0264-410X
DOI: 10.1016/j.vaccine.2007.10.047
Appears in Collections:期刊論文

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