Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/71566
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dc.contributor.authorSu, B.S.en_US
dc.contributor.authorYin, H.S.en_US
dc.contributor.authorChiu, H.H.en_US
dc.contributor.authorHung, L.H.en_US
dc.contributor.authorHuang, J.P.en_US
dc.contributor.authorShien, J.H.en_US
dc.contributor.authorLee, L.H.en_US
dc.date2011zh_TW
dc.date.accessioned2014-06-11T06:01:58Z-
dc.date.available2014-06-11T06:01:58Z-
dc.identifier.issn0378-1135zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/71566-
dc.description.abstractRecombinant fowlpox virus (rFPV/HN) expressing Newcastle disease virus (NDV) HN gene and rFPV/HN/chIL-12 co-expressing chicken IL-12 (chIL-12) and HN (rHN/chIL-12) genes have been characterized. rHN/chIL-12 or rchIL-12, expressed by our previous construct rFPV/chIL-12, co-administered with rHN was assessed for adjuvant activities of chIL-12. Chickens were vaccinated with various amounts of rHN/chIL-12 mixed with mineral oil (MO), intramuscularly. Levels of hemagglutination-inhibition (HI) antibody production depended on the concentration of the injected rHN or rHN/chIL-12. The lower HI antibody titers were obtained in chicken groups rHN/chIL-12/7-rHN/chIL-12/9, receiving 60 ng rHN/8 ng chIL-12 with MO, 30 ng rHN/4 ng chIL-12 with MO or 15 ng rHN/2 ng chIL-12 with MO, respectively, compared to those in chicken groups rHN/7-rHN/9, receiving rHN with MO alone. However, chickens in group rHN/chIL-12/7 or rHN/chIL-12/8 and rHN with MO alone showed the same effective protection. Chicken group rHN/chIL-12/9 was even more protective than that in group rHN/9. When rchIL-12 was co-injected with 15 ng rHN plus MO, chickens produced low levels of HI antibody titers; while higher levels of IFN-gamma production and an effective protection rate (83%) were obtained. On the other hand, low levels of IFN-gamma production and low protection response (50%) were obtained in chickens injected with rHN with MO alone. Taken together, when the concentration of rHN decreased to certain levels, rchIL-12 reduced HI antibody production. The increase in the induction of IFN-gamma production might suggest the enhancement of the cell-mediated immunity which conferred the protection from the NDV challenge. (C) 2011 Elsevier B.V. All rights reserved.en_US
dc.language.isoen_USzh_TW
dc.relationVeterinary Microbiologyen_US
dc.relation.ispartofseriesVeterinary Microbiology, Volume 151, Issue 3-4, Page(s) 220-228.en_US
dc.relation.urihttp://dx.doi.org/10.1016/j.vetmic.2011.02.047en_US
dc.subjectChicken IL-12en_US
dc.subjectNDen_US
dc.subjectHN proteinen_US
dc.subjectFPVen_US
dc.subjectRecombinanten_US
dc.subjectinterferon-gammaen_US
dc.subjectin-vivoen_US
dc.subjectescherichia-colien_US
dc.subjecthumoral immunityen_US
dc.subjectvillageen_US
dc.subjectchickensen_US
dc.subjectfowlpox virusesen_US
dc.subjectavian cytokinesen_US
dc.subjectstrain i-2en_US
dc.subjectifn-gammaen_US
dc.subjectt-cellsen_US
dc.titleImmunoadjuvant activities of a recombinant chicken IL-12 in chickens vaccinated with Newcastle disease virus recombinant HN proteinen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1016/j.vetmic.2011.02.047zh_TW
item.fulltextno fulltext-
item.languageiso639-1en_US-
item.openairetypeJournal Article-
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextnone-
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