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標題: Immunoadjuvant activities of a recombinant chicken IL-12 in chickens vaccinated with Newcastle disease virus recombinant HN protein
作者: Su, B.S.
Yin, H.S.
Chiu, H.H.
Hung, L.H.
Huang, J.P.
Shien, J.H.
Lee, L.H.
關鍵字: Chicken IL-12;ND;HN protein;FPV;Recombinant;interferon-gamma;in-vivo;escherichia-coli;humoral immunity;village;chickens;fowlpox viruses;avian cytokines;strain i-2;ifn-gamma;t-cells
Project: Veterinary Microbiology
期刊/報告no:: Veterinary Microbiology, Volume 151, Issue 3-4, Page(s) 220-228.
Recombinant fowlpox virus (rFPV/HN) expressing Newcastle disease virus (NDV) HN gene and rFPV/HN/chIL-12 co-expressing chicken IL-12 (chIL-12) and HN (rHN/chIL-12) genes have been characterized. rHN/chIL-12 or rchIL-12, expressed by our previous construct rFPV/chIL-12, co-administered with rHN was assessed for adjuvant activities of chIL-12. Chickens were vaccinated with various amounts of rHN/chIL-12 mixed with mineral oil (MO), intramuscularly. Levels of hemagglutination-inhibition (HI) antibody production depended on the concentration of the injected rHN or rHN/chIL-12. The lower HI antibody titers were obtained in chicken groups rHN/chIL-12/7-rHN/chIL-12/9, receiving 60 ng rHN/8 ng chIL-12 with MO, 30 ng rHN/4 ng chIL-12 with MO or 15 ng rHN/2 ng chIL-12 with MO, respectively, compared to those in chicken groups rHN/7-rHN/9, receiving rHN with MO alone. However, chickens in group rHN/chIL-12/7 or rHN/chIL-12/8 and rHN with MO alone showed the same effective protection. Chicken group rHN/chIL-12/9 was even more protective than that in group rHN/9. When rchIL-12 was co-injected with 15 ng rHN plus MO, chickens produced low levels of HI antibody titers; while higher levels of IFN-gamma production and an effective protection rate (83%) were obtained. On the other hand, low levels of IFN-gamma production and low protection response (50%) were obtained in chickens injected with rHN with MO alone. Taken together, when the concentration of rHN decreased to certain levels, rchIL-12 reduced HI antibody production. The increase in the induction of IFN-gamma production might suggest the enhancement of the cell-mediated immunity which conferred the protection from the NDV challenge. (C) 2011 Elsevier B.V. All rights reserved.
ISSN: 0378-1135
DOI: 10.1016/j.vetmic.2011.02.047
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