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|標題:||Pulsed field gel electrophoresis for animal Salmonella enterica serovar Typhimurium isolates in Taiwan||作者:||Tsen, H.Y.
|關鍵字:||Salmonella serovar Typhimurium;PFGE;serotype typhimurium;phage types;enteritidis;pfge;humans||Project:||Veterinary Microbiology||期刊/報告no：:||Veterinary Microbiology, Volume 87, Issue 1, Page(s) 73-80.||摘要:||
Salmonella enterica subspecies enterica serovar Typhimurium is a common pathogen for humans and animals. In order to trace the clonal relationship and to find the circulating strains between human and animal isolates, chromosomal DNAs from 87 serovar Typhimurium strains isolated from animals (pigs were the majority) were subjected to XbaI and SpeI digestion and pulsed field gel electrophoresis (PFGE). For the 87 animal isolates, 38 PFGE pattern combinations were obtained. As the subtyping results from animal isolates were compared with those from the 45 human isolates, it was found that 14 of the animal isolates and 13 of the human isolates shared a common PFGE pattern combination, i.e., pattern XgSf (or called X5S4). When these human and animal isolates were subjected to antibiotic susceptibility test using I I antibiotics, it was found that strains of pattern XgSf (X5S4) belong to a common antibiogram pattern which is tetracycline, gentamicin, ampicillin, streptomycin and chloramphenicol resistant. Since most of the animal and human strains in pattern XgSf were originally isolated from various areas over different years, strains of this PFGE pattern may be the most epidemic strains which circulating between human and animal sources. (C) 2002 Elsevier Science B.V. All rights reserved.
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