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標題: Genome sequence of the lytic bacteriophage P1201 from Corynebacterium glutamicum NCHU 87078: Evolutionary relationships to phages from Corynebacterineae
作者: Chen, C.L.
Pan, T.Y.
Kan, S.C.
Kuan, Y.C.
Hong, L.Y.
Chiu, K.R.
Sheu, C.S.
Yang, J.S.
Hsu, W.H.
Hu, H.Y.
關鍵字: Corynebacterium glutamicum;corynephage;lytic phage;complete genome;sequence;sequence analysis;brevibacterium-flavum;tail length;proteins;identification;purification;acid
Project: Virology
期刊/報告no:: Virology, Volume 378, Issue 2, Page(s) 226-232.
P1201 is a lytic corynephage of Corynebacterium glutamicum NCHU 87078. Its genome consists of a linear double-stranded DNA molecule of 70,579 base pairs, with 3'-protruding cohesive ends often nucleotides. We have identified 69 Putative open reading frames, including three apparent genes (thymidylate synthase, terminase, and RNR alpha subunit genes) that are interrupted by an intein. Protein-splicing activities of these inteins were demonstrated in Escherichia coli. Three structural proteins including major capsid and major tail proteins were separated by SDS-PAGE and identified by both LC-MS-MS and N-terminal sequence analyses. Bioinformatics analysis indicated that only about 8.7% of its putative gene products shared Substantial protein sequence similarity with the lytic corynephage BFK20 from Brevibacterium flavum, the only corynephage whose genome had been sequenced to date, revealing that the P1201 genome is distinct from BFK20. The mosaic-like genome of P1201 indicates extensive horizontal gene transfer among P1201, Gordonia terrae phage GTE5, mycobacteriophages, and several regions of Corynebacterium spp. genomes. (C) 2008 Elsevier Inc. All rights reserved.
ISSN: 0042-6822
DOI: 10.1016/j.virol.2008.05.027
Appears in Collections:期刊論文

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