Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/71576
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dc.contributor.authorYeh, T.Y.en_US
dc.contributor.authorLin, B.Y.en_US
dc.contributor.authorChang, Y.C.en_US
dc.contributor.authorHsu, Y.H.en_US
dc.contributor.authorLin, N.S.en_US
dc.date1999zh_TW
dc.date.accessioned2014-06-11T06:02:00Z-
dc.date.available2014-06-11T06:02:00Z-
dc.identifier.issn0920-8569zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/71576-
dc.description.abstractA naturally occurring 1.1 kb RNA was isolated from purified virions of bamboo mosaic potexvirus isolate S (BaMV-S). This RNA is a defective RNA (D RNA) derived from a single internal deletion of the BaMV genome. A cDNA clone representing the complete nucleotide sequence of the BaMV-S D RNA was generated and its nucleotide sequence was determined. The BaMV D cDNA is 1015 nts in length [excluding the poly(A) tail] and consists of two regions corresponding to 867 nts of the 5' terminus and 148 nts of the 3' terminus of the BaMV genomic RNA. BaMV D cDNA contains a single open reading frame (ORF) encoding a putative 29.7 kDa protein comprised of a fusion of the first 258 amino acids of BaMV ORF 1 and the last 2 amino acids of coat protein. The coding capacity of D RNA was verified by in vitro translation of native BaMV-S D RNA and of 1.1 kb RNA transcribed in vitro from the full-length D cDNA. BaMV D RNA can be reproducibly generated by serial passages of BaMV-S in Nicotiana benthamiana and is the first D RNA in the potexvirus group shown to be generated de novo. Alignments of sequences surrounding the 5' and 3' junction borders of reported potexvirus D RNAs reveal a 65.2-84.6% sequence identity, suggesting that common mechanisms for viral RNA recombination are involved in the generation of potexvirus D RNAs.en_US
dc.language.isoen_USzh_TW
dc.relationVirus Genesen_US
dc.relation.ispartofseriesVirus Genes, Volume 18, Issue 2, Page(s) 121-128.en_US
dc.relation.urihttp://dx.doi.org/10.1023/a:1008008400653en_US
dc.subjectbamboo mosaic potexvirusen_US
dc.subjectdefective RNAen_US
dc.subjectRNA combinationen_US
dc.subjectbean mottle bromovirusen_US
dc.subjectinterfering rnasen_US
dc.subjectdenovo generationen_US
dc.subjectinvivoen_US
dc.subjectaccumulationen_US
dc.subjectnucleotide-sequenceen_US
dc.subjectcoding capacityen_US
dc.subjectsatellite rnaen_US
dc.subjectreplicationen_US
dc.subjectinfectionsen_US
dc.subjectproteinen_US
dc.titleA defective RNA associated with bamboo mosaic virus and the possible common mechanisms for RNA recombination in potexvirusesen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1023/a:1008008400653zh_TW
item.grantfulltextnone-
item.openairetypeJournal Article-
item.languageiso639-1en_US-
item.fulltextno fulltext-
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
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