Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/71583
DC FieldValueLanguage
dc.contributor.authorWong, M.L.en_US
dc.contributor.authorYen, Y.R.en_US
dc.date1998zh_TW
dc.date.accessioned2014-06-11T06:02:01Z-
dc.date.available2014-06-11T06:02:01Z-
dc.identifier.issn0168-1702zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/71583-
dc.description.abstractThe impact of pseudorabies virus (PRV) infection on the synthesis of host cell proteins was studied. By metabolic labeling of protein synthesis with [S-35]methionine, it vias observed that the translation of cellular proteins was inhibited globally in the late phase of infection and viral proteins became the dominating products. Furthermore, immunoblot analysis showed that the total protein levels of two genes involved in translational regulation, namely the dsRNA-dependent protein kinase (PKR) and extracellular signal-regulated kinase 2 (ERK2), decreased during late time of infection. Using [P-32]orthophosphate labeling, it was observed that PRV infection also caused a decrease in the phosphorylation of intracellular PKR. Finally, using 2-aminopurine (2-AP, an inhibitor of serine/threonine protein kinase) or adenine tan isomer of 2-AP) to treat PRV-infected cells, we found that the inhibition of host protein synthesis by PRV was partially prevented by these two drugs, suggesting that 2-AP and adenine may share a same target and pathway to manifest the effect. (C) 1998 Elsevier Science B.V. All rights reserved.en_US
dc.language.isoen_USzh_TW
dc.relationVirus Researchen_US
dc.relation.ispartofseriesVirus Research, Volume 56, Issue 2, Page(s) 199-206.en_US
dc.relation.urihttp://dx.doi.org/10.1016/s0168-1702(98)00077-xen_US
dc.subjectpseudorabies virusen_US
dc.subjectprotein synthesisen_US
dc.subjectdouble-stranded-rnaen_US
dc.subjectgene-expressionen_US
dc.subjectmessenger-rnaen_US
dc.subjecttopoisomerase-iien_US
dc.subjectbinding domainsen_US
dc.subjectinterferonen_US
dc.subjecttranslationen_US
dc.subjectinhibitionen_US
dc.subjectinitiationen_US
dc.subjectphosphorylationen_US
dc.titleProtein synthesis in pseudorabies virus-infected cells: decreased expression of protein kinase PKR, and effects of 2-aminopurine and adenineen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1016/s0168-1702(98)00077-xzh_TW
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
item.cerifentitytypePublications-
item.fulltextno fulltext-
item.languageiso639-1en_US-
item.grantfulltextnone-
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