Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/71603
DC FieldValueLanguage
dc.contributor.authorYamagata, T.en_US
dc.contributor.authorIshii, M.en_US
dc.contributor.authorNarita, M.en_US
dc.contributor.authorHuang, G.C.en_US
dc.contributor.authorEndo, G.en_US
dc.date2002zh_TW
dc.date.accessioned2014-06-11T06:02:05Z-
dc.date.available2014-06-11T06:02:05Z-
dc.identifier.issn0273-1223zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/71603-
dc.description.abstractBioluminescence sensor systems were developed for monitoring environmental mercury contamination. The biological mercury measurement sensor systems were constructed by DNA recombination technique. A bacterial mercury-resistant operon (mer operon) from Pseudomonas sp. K-6y4 and a bacterial bioluminescence operon (lux operon) from an ocean bacterium Vibrio fischeri were fused in a vector plasmid. The resulting recombinant plasmids were cloned in Escherichia coli cells. The bioluminescence sensor systems responded to mercury chloride of 0.1 nM to 100 nM. The mercury bioluminescence sensor developed in this study can be used-for monitoring of the bio-affecting mercury instead of total mercury that is measured by conventional analytical,equipment. The fundamental feature,of the bioluminescence sensor system is attractive for use as a monitoring system for bio-affecting environmental mercury contamination.en_US
dc.language.isoen_USzh_TW
dc.relationWater Science and Technologyen_US
dc.relation.ispartofseriesWater Science and Technology, Volume 46, Issue 11-12, Page(s) 253-256.en_US
dc.subjectbioluminescence sensoren_US
dc.subjectgenetic recombinationen_US
dc.subjectlux operonen_US
dc.subjectmer operonen_US
dc.subjectmercury sensoren_US
dc.subjectmonitoringen_US
dc.subjectpollutionen_US
dc.subjectamazonen_US
dc.subjectbrazilen_US
dc.titleBio-affecting mercury detection using mercury resistance gene module fused with bioluminescence reporter genesen_US
dc.typeJournal Articlezh_TW
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
item.cerifentitytypePublications-
item.fulltextno fulltext-
item.languageiso639-1en_US-
item.grantfulltextnone-
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