Please use this identifier to cite or link to this item:
|標題:||Molecular identification of two sibling species of Puntius in Taiwan||作者:||Chang, C.H.
|關鍵字:||cytochrome b gene;freshwater fish;molecular clock;phylogenetic tree;animal mitochondrial-dna;evolution;substitution;phylogeny;clock||Project:||Zoological Studies||期刊/報告no：:||Zoological Studies, Volume 45, Issue 2, Page(s) 149-156.||摘要:||
Puntius fish from Taiwan and South China were collected and analyzed. Specimens from the northern and central Taiwan were characterized by the absence of barbels. On the contrary, specimens from the southern Taiwan and South China were characterized by the presence of barbels. These characteristics morphologically match P. snyderi and P. semifasciolatus respectively (Oshima 1919). We hypothesized that there were two species of Puntius in Taiwan. To test this hypothesis, we amplified and sequenced the cytochrome b gene from fish specimens of China and Taiwan. Phylogenetic analysis revealed the existence of two major clades of Puntius fishes with an average genetic distance of 0.12 between them. The fish specimens from southern Taiwan were clustered with P. semifasciolatus of China, but fish specimens from northern and central Taiwan were clustered together. The estimated evolutionary rate of cytochrome b gene for Puntius was 0.368% per million yrs (MY). Thus the divergence time between P. snyderi and P. semifasciolatus was about 26.93 million years ago (MYA) and the divergent time between Taiwan's and China's P. semifasciolatus was about 4.40 MYA. Taken together, our results supported the existence of two species of Puntius fishes in Taiwan. P. snyderi is distributed in northern and central Taiwan, while P. semifasciolatus is in southern Taiwan.
|Appears in Collections:||期刊論文|
Show full item record
TAIR Related Article
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.