Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/71656
DC FieldValueLanguage
dc.contributor.authorJayaraman, G.en_US
dc.contributor.author系統管理者zh_TW
dc.contributor.authorKumar, T.K.S.en_US
dc.contributor.authorTsai, C.C.en_US
dc.contributor.authorSrisailam, S.en_US
dc.contributor.authorChou, S.H.en_US
dc.contributor.authorHo, C.L.en_US
dc.contributor.authorYu, C.en_US
dc.contributor.author周三和zh_TW
dc.date2000zh_TW
dc.date.accessioned2014-06-11T06:02:12Z-
dc.date.available2014-06-11T06:02:12Z-
dc.identifier.issn0961-8368zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/71656-
dc.description.abstractThe aim of the present study is to understand the structural features responsible for the lethal activity of snake venom cardiotoxins. Comparison of the lethal potency of the five cardiotoxin isoforms isolated from the venom of Taiwan cobra (Naja naja atra) reveals that the lethal potency of CTX I and CTX V are about twice of that exhibited by CTX II, CTX III, and CTX IV. In the present study, the solution structure of CTX V has been determined at high resolution using multidimensional proton NMR spectroscopy and dynamical simulated annealing techniques. Comparison of the high resolution solution structures of CTX V with that of CTX IV reveals that the secondary structural elements in both the toxin isoforms consist of a triple and double-stranded antiparallel beta-sheet domains. Critical examination of the three-dimensional structure of CTX V shows that the residues at the tip of Loop III form a distinct "finger-shaped" projection comprising of nonpolar residues. The occurrence of the nonpolar "finger-shaped" projection leads to the formation of a prominent cleft between the residues located at the tip of Loops II and III. Interestingly, the occurrence of a backbone hydrogen bonding (Va127CO to Leu48NH) in CTX IV is found to distort the "finger-shaped" projection and consequently diminish the cleft formation at the tip of Loops II and III. Comparison of the solution structures and lethal potencies of other cardiotoxin isoforms isolated from the Taiwan cobra (Naja naja atra) venom shows that a strong correlation exists between the lethal potency and occurrence of the nonpolar "finger-shaped" projection at the tip of Loop III. Critical analysis of the structures of the various CTX isoforms from the Taiwan cobra suggest that the degree of exposure of the cationic charge (to the solvent) contributed by the invariant lysine residue at position 44 on the convex side of the CTX molecules could be another crucial factor governing their lethal potency.en_US
dc.language.isoen_USzh_TW
dc.relationProtein Scienceen_US
dc.relation.ispartofseriesProtein Science, Volume 9, Issue 4, Page(s) 637-646.en_US
dc.subjectcardiotoxinsen_US
dc.subjectfinger-shaped projectionen_US
dc.subjectlethal potencyen_US
dc.subjectreceptoren_US
dc.subjectbindingen_US
dc.subjectsolution structureen_US
dc.subjectchemical modificationen_US
dc.subjectnmr-spectroscopyen_US
dc.subjectamino-aciden_US
dc.subjectproteinen_US
dc.subjectneurotoxinsen_US
dc.subjectsequenceen_US
dc.subjectdynamicsen_US
dc.subjectresiduesen_US
dc.subjectspectraen_US
dc.subjectclbpen_US
dc.titleElucidation of the solution structure of cardiotoxin analogue V from the Taiwan cobra (Naja naja atra) - Identification of structural features important for the lethal action of snake venom cardiotoxinsen_US
dc.typeJournal Articlezh_TW
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextno fulltext-
item.grantfulltextnone-
item.cerifentitytypePublications-
item.languageiso639-1en_US-
item.openairetypeJournal Article-
Appears in Collections:期刊論文
Show simple item record
 

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.