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標題: 建立高效液相層析技術以分析與純化生物界面活性劑之方法
作者: 陳億全
關鍵字: 生物界面活性劑;高效液相層析;超過濾;臨界微胞濃度
出版社: 國立中興大學工學院;Airiti Press Inc.
Project: 興大工程學報, Volume 10, Issue 1, Page(s) 25-33.
A general approach for the development of high-performance liquid chromatography(HPLC) methods for biosurfactants analysis and purification was proposed. By comparing the chromatograms of the cell-free fermentation broth, the ultrafiltration filtrate, and the ultrafiltration filtrate of methanol-surfactant mixture, the peaks corresponding to biosurfactants can be identified without any prior structural information of the biosurfactants. It can be assumed that the peaks observed only on the chromatogram of the filtrate of metanhol-surfactant mixture but not on the chromatogram of the filtrate are biosurfactant peaks. This approach can be applied to the development of ITPLC assay for any biosurfactants as long as the concentration of biosurfactants in the fermentation broth is higher than the critical micelle concentration(CMC). This HPLC methods thus developed can also be adapted for the preparation of homogeneous biosuirfactant samples useful for chemical analysis for the elucidation of chemical structure of biosurfactants and for the determination of the physical properties of biosurfactants.

ISSN: 1017-4397
Appears in Collections:第10卷 第1期

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