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標題: 探討紅麴菌二次代謝物生產之研究:(I)紅麴天然色素
作者: 賴龍山
關鍵字: 紅麴菌;紅麴色素生產;培養基設計
出版社: 國立中興大學工學院;Airiti Press Inc.
Project: 興大工程學刊, Volume 14, Issue 2, Page(s) 97-105.
The present study was to investigate the speculation and optimize the production medium for pigment production by Monascus pilosus ATCC 22080 in shake-flask experiments. The medium containing 2% maltose and 0.1% peptone was found to aid its sporulation. In the work, pre-cultures was used to induce mycelium growth, which was then transferred to produce Monascus-pigments. The Plackett-Burma method and two-level factorial designs were used to screen the important ingredients, followed by the method of path of the steepest ascent and response surface methodology. Using a 3-factor-5-level central composite design, the system response was optimized at: Glycerol, 32.9; Maltos, 84.4; KH2PO4, 13.8; glucose, 3; MSG, 12.5; NH4NO3, 1.5. The process for Monascus-pigment production was thus significantly shortened. Such experiments described that maltose was the best carbon source, and a high concentration of maltose greatly enhanced product formation.

本研究以紅麴菌Monascus pilosus ATCC 22080為紅麴色素生產菌株,碳源豐富而氮源限制有助於此菌株之產孢,實驗中並以搖瓶實驗探討生產培養基之最適化。本文是接種前培養菌絲來生產天然色素,實驗上利用Plackett-Burman實驗設計及兩水準部分因子設計法先篩選出重要的組成分,配合陡昇路徑與回應曲面法尋求極值點,3因子5水準的中心混成設計之實驗結果顯示最適化生產培養基為(g/L):Glycerol,32.9;Maltose,84.4;KH2PO4,13.8;Gluscose,3;MSG,12.5;與NH4NO3,1.5;相較最適化之前,紅麴色素之生產由本研究初之十天大幅縮短為四天;實驗數據說明Maltose為最佳碳源,也說明了高濃度Maltose有助於紅麴色素之生產。
ISSN: 1017-4397
Appears in Collections:第14卷 第2期

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