Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/81516
標題: Studies on Shoot Tip Culture of Passionfruit in Vitro
百香果莖頂組織培養之研究
作者: Tsong-An Yu
翁慎微
Shen-Wei Weng
Yau-Shiang Yang
楊耀祥
余聰安
關鍵字: Shoot tip culture;莖頂培養;Passionfruit;百香果
Project: Horticulture NCHU, Volume 18, Page(s) 29-44.
興大園藝, Volume 18, Page(s) 29-44.
摘要: 
本研究係利用組織培養之技術,採取百香果莖頂生長點加以培養,調查影響其成活因子及探求各培養時期最適當的培養方法,期以此結果建立百香果莖頂培養之技術,以供健康苗木生產之參考。
在新梢生長旺盛其之春季,取莖頂生長點(0.2-0.3mm大小)培植於1/2 MS添加0.02mg/l IBA、0.2mg/l BA、0.5mg/l GA3及30g/l蔗糖之濾紙液體培養基,培養30日後有100%的成活率,再移入相同的固態培養基,經2-3次的繼代培養,即有側芽之發生。
將長5mm的芽體培養於含0.05mg/l IBA、0.1mg/l BA及0.5mg/l GA3 之培養培養基中,培養30日後可得4.3倍 1 cm以上的增殖芽體。
將增殖培養1 cm以上的枝梢,以2000ppm IBA處理後,扦插於蛭石、真珠石及泥炭苔之混合介質中,經30日後,有100%發根率及最佳的發根數與根長,該發根效果較瓶內發根為佳。

The effects of cultural media and methods on the survival rate shoot tips of Passiflora edulis Sims in vitro were studied, and the mass multiplication technique and procedure were established,
Soot tips were harvested about 3 cm in length from actively potted-grown plants in spring and shoots apices were excised 0.2-0.3 mm for intial culture. The survival rate reached 100% when shoot apices cultured on the paper bridge medium with 1/2 strenth of Murashige and Skoog (MS) containing 0.02 mg/l indol-3-butyric acid (IBA), 0.2 mg/l 6-benzyladenin (BA), 0.5 mg/l gibberillin acid (GA3) and 30 g/l sucrose for 30 days, and then these explants were transfered to the same component soild medium (added 10g/l agar) for 2-3 subcultures in order to formate lateral buds.
Multiple shoots were induced by 1/2 MS medium with 0.05 mg/l IBA, 0.1 mg/l IBA and 0.5 mg/l GA3. Each subculture could be harvested 4.3 multipliable shoots (5 mm) within 30 days.
When shoots (1 cm) were treated with 200ppm IBA and cutting in the mixture of vermiculite, paerlite and peatmoss, the rooting rate of these cuttings could be reached 100% after cutting for 30 days.
URI: http://hdl.handle.net/11455/81516
ISSN: 0255-5921
Appears in Collections:園藝學系
第18期

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