Please use this identifier to cite or link to this item:
標題: 共同轉移蘇力菌殺蟲晶體蛋白、D型胺基酸氧化酵素、轉酮醇酵素、熱休克蛋白等基因至甘藍之研究
Studies on Co-transfer of Bt-toxin, D-Amino Acid Oxidase, Transketolase, and Heat Shock Protein into Cabbage via Agrobacterium
作者: 黃群益
Chun-I Huang
Menq-Jiau Tseng
關鍵字: Co-transfermation;農桿菌共同轉移法;Bt-toxin;D-amino acid oxidase;Transketolase;Heat shock protein;蘇力菌殺晶體蛋白;D型胺基酸氧化酵素;轉酮醇酵素;熱休克蛋白;基因
Project: Horticulture NCHU, Volume 24, Issue 4, Page(s) 41-58.
興大園藝, Volume 24, Issue 4, Page(s) 41-58.

Attempts had been made to co-transfer the Bt-toxin (Bt), D-amino acid oxidase (DAO), transketolase (TKL), and heat shock protein (HSP) genes into the cabbage. The objectives of this study are to establish the gene co-transfer technology and to study the possibility for improvement of cabbage with insect, disease, and stresses resistance by gene transformation.
Regenerated plants were obtained after co-transformation with four kinds of genes (Bt + DAO + TKL + HSP) into the cabbage and screened by Southern slot hybridization. The transgenic plants were further examined by PCR and Northern hybridization. Several transgenic plants contained four, three, and two kinds of genes in one plant were inserted into the chromosome of transgenic plants and expressed Bt transcript with 1.35 kb RNA, DAO transcript with 1.1 kb RNA, TKL transcript with 2.2 kb RNA, HSP transcript with 2.1 kb RNA. The study demonstrated that two, three and four foreign genes could be co-transferred into cabbage plant simultaneously via Agrobacterium mediated transformation.
ISSN: 0255-5921
Appears in Collections:園藝學系
第24卷 第04期

Files in This Item:
File Description SizeFormat Existing users please Login
興大園藝第二十四卷第四期p041~p058.pdf3.88 MBAdobe PDFThis file is only available in the university internal network    Request a copy
Show full item record

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.