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Study on Endo-B-mannanase and Ethylene in Lettuce (Lactuca sativa L.) Seed Germination Matured under Different Temperature
|關鍵字:||Endo-B-mannanase;種子發芽溫度;Seed matured temperature;Lettuce seed;Ethylene;萵苣種子;乙烯||Project:||興大園藝, Volume 28, Issue 1, Page(s) 55-70.
Horticulture NCHU, Volume 28, Issue 1, Page(s) 55-70.
'Dark Green Boston' 與 'Everglade' 30/20℃發育的種子較20/10℃發育者分別於發芽限溫27℃或33℃之發芽率較高，兩品種於30/20℃發育種子於高溫下酵素活性均高於20℃十表現，萵苣種子珠孔區胚乳或側邊胚乳於30/20℃或20/10℃發育者，所含endo-β-mannanase活性與發芽率呈正相關，胚根突出後此酵素活性大量產生。內生乙烯生合成隨發芽率增加而增加，'Dark Green Boston' 與 'Everglade' 之30/20℃發育種子於高溫時乙烯產生量較20/10℃者多。
The germination of ‘Dark Green Boston' and ‘Everglade' seeds matured at 30/20℃ was higher than the seeds matured at 20/10℃. The germination of ‘Dark Green Boston' and ‘Everglade' seeds matured at 30/20℃ was 97.8% and 52.2% at 27℃ or 33℃, respectively. The mean germination time of seeds matured at 30/20℃ were shortened compared to the seeds matured at 20/10℃. A gel-diffusion assay was used to measure endo-β-mannanase activity during the germination of lettuce seed. At 27℃ or 33℃ befer radicle protrusion, endo-β-mannanase activity at micropylar region of ‘Dark Green Boston' and ‘Everglade' matured at 30/20℃ seeds were higher than those matured at 20/10℃. The evolution of ethylene from ‘Dark Green Boston' and ‘Everglade' matured at 30/20℃ seeds were higher than those matured at 20/10℃. As the germination percentage increased, ethylene production from seeds was increased. The amount of ethylene produced from seeds germinated at 27℃ was more than that at 20℃. The role of the ethylene production and endo-β-mannanase activity before radicle protrusion when the seed germination at high temperature was confirmed in this study.
|Appears in Collections:||第28卷 第01期|
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