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標題: Studies on Proliferation from Anther Culture of Gladiolus hybridus Hort.
作者: 周思潔
Su-Jie Chou
Tsai-Yih Wang
關鍵字: 馴化處理;Acclimatization;品種;癒合組織;培養基成分;花藥培養;Cultivar;Callus;Anther culture;Media composition
Project: Horticulture NCHU, Volume 28, Issue 1, Page(s) 103-114.
興大園藝, Volume 28, Issue 1, Page(s) 103-114.
以唐菖蒲(Gladiolus hybridus Hort.)三個商業栽培品種進行花要培養試驗,探討瓶內培養的%養雞成分對癒合組織增殖與芽體分化之影響,以及值值瓶外的馴化處理對小苗生長之影響。唐菖蒲'Princess Margaret Rose'品種於不同培養基中所得愈合組織誘導率為最佳(分別為15%及20%)。在0.1mg/l 2,4-D 配合0.5mg/l Kinetin 或0.5mg/l NAA配合0.1或0.5mg/l BA之培養基在癒合組織增值率及芽體再生能力上有最佳的表現;另在培養基中添加0.3-0.5%PDA有提高芽體與小苗再生率。瓶苗移植於高相對溼度(RH85%)、28/18(日溫/夜溫)的環境中,配合開平3-5天漸進的降低瓶內濕度,可提高瓶苗移植後的存活率。

Anthers of three commercial cultivars of Gladiolus hybridus Hort. Were used, to understand the effect of media composition on proliferation of calli and differentiation of shoots during in vitro culture, and the effect of acclimatization treatments on plantlets growth during in vitro culture, and the effect of acclimatization treatments on plantlets growth during in vivo were of grest interest. ‘Princess Margaret Rose' cultured under each medium gave the highest rate of calli (15.0% and 20.0%). The calli were cultured on 1/2MS medium with 0.1mg/l 2,4-D and 0.5mg/l Kinetin or 0.5mg/l NAA and 0.1 or 0.5mg/l BA showed that with was the best rate of calli proliferation and shoots regeneration. In order to enhance the regeneration rate of shoots and plantlets, it could be supplemented with 0.3-0.5% PDA in the medium. The plantlets transplant in the environment that had high relative humidity (RH85%) and 28/18℃(day/night) temperature, to be operated in three days of opening container lip to progress step by step to reduce the vessel humidity, could enhance the survival rate of transplants.
ISSN: 0255-5921
Appears in Collections:第28卷 第01期

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