Auxins induce somatic Embryogenesis via In Vitro Culture of Leaflet Explants of Roses

Abstract

For increasing embryogenic calli formation rate and reducing time of somatic embryogenesis therefore. A procedure for plant regeneration leaflets of roses (Rosa hybrida L.) via in vitro culture is described. Two auxins, NAA and picloram alone, at various concentrations(0.25, 0.5, 1 or 2 mg/l) were tested for its capacity to induce somatic embryogenesis from in vitro- derived leaflet of five cultivars (Christian Dior, Tiffany, Oklahome, Laser, White Queen Elizabeth). Calli could be initiated from in vitro leaflet of five rose cultivars on Murashige and Skoog (MS) medium with NAA or picloram. Although all rose cultivars could from calli, but only ‘Tiffany'could produce embryogenic calli on MS medium including NAA(1 or 2 mg/l).The influence of genetype on embryogenic differentiation is remarkable. In auxins experiment, leaflet explants were precultured on MS medium with 1 mg/l NAA for four weeks followed by subculture on MS medium with 2 mg/l BA. ‘Tiffany'showed the highest amount of regeneration plants.

為了提高胚性癒傷組織的形成率並縮短體胚發生的時間，因此描述一種從玫瑰花器內小葉經由體胚發生再生植株的步驟。測試NAA和picloram兩種生長素在不同濃度(0.2L0.5,1 or 2 mg/l)下對五個品種玫瑰花(克利斯汀 迪奧、鐵凡尼、奧克拉荷馬、雷射、白英國女王)的器內小葉培植體誘導體胚發生的能力。在含NAA 或 picloram的MS培養基中，癒傷組織可從五種玫瑰花品種的器內小葉中被產生。雖然所有品種都能產生癒傷組織，但是僅'鐵凡尼'能在含NAA的MS培番基產生胚性癒傷組織。基因型在胚性分化上的影響顯著。'鐵凡尼'小葉培植體培養在含lmg/l NAA的MS培養基4週後，再繼代培養到含2mg/l BA的MS培養基，再生株數量最高。